College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
Bioresour Technol. 2010 Jun;101(12):4564-9. doi: 10.1016/j.biortech.2010.01.113. Epub 2010 Feb 13.
Response surface methodology was used to optimize extraction parameters for Morchella esculenta SO-01 exopolysaccharide (EPS) produced during submerged culture. The optimum conditions for EPS extraction were predicted to be, concentration at 84.07 degrees C, precipitation for 22.19 h and pH 8.44, and EPS production was estimated at 5.45 g/L. The actual yield of EPS under these conditions was 5.32 g/L. The antioxidant capacity of the EPS was measured in vivo after filling the stomach with different doses of EPS and results showed that the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) of blood, spleen, liver, heart, and kidney were increased by 125%, 46.11%, 23.33%, 12.19%, 41.29%, and 63.24%, 63.12%, 166.54%, 98.01%, 57.68%, respectively, and that malonaldehyde (MDA) of blood, spleen, liver, heart, and kidney were decreased by 21.80%, 67.84%, 28.48%, 56.15%, 41.62%. The results provide a reference for large-scale extraction of EPS by M. esculenta SO-01 in industrial fermentation.
响应面法被用于优化 Morchella esculenta SO-01 胞外多糖（EPS）的浸提工艺参数。预测 EPS 提取的最佳条件为：浓度 84.07°C，沉淀 22.19 h，pH 8.44，EPS 产量估计为 5.45 g/L。在此条件下，EPS 的实际产量为 5.32 g/L。通过向胃中填充不同剂量的 EPS 来测量 EPS 的体内抗氧化能力，结果表明，血液、脾脏、肝脏、心脏和肾脏中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性分别提高了 125%、46.11%、23.33%、12.19%、41.29%和 63.24%、63.12%、166.54%、98.01%、57.68%，血液、脾脏、肝脏、心脏和肾脏中的丙二醛（MDA）分别降低了 21.80%、67.84%、28.48%、56.15%、41.62%。这些结果为 M. esculenta SO-01 在工业发酵中大规模提取 EPS 提供了参考。
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