Song Zhen, Jia Le, Xu Feng, Meng Fanyun, Deng Peng, Fan Keming, Liu Xiaonan
College of Life Sciences, Shandong Agricultural University, Taian, Shandong, China.
Biol Trace Elem Res. 2009 Oct;131(1):81-9. doi: 10.1007/s12011-009-8343-8. Epub 2009 Feb 28.
Selenium (Se) is an essential trace element for humans and animals. Stropharia rugoso-annulata is a nutritional and functional mushroom containing many kinds of bioactive ingredients. The aims of this study were to investigate the Se-enrichment characteristics of S. rugoso-annulata in submerged culture and evaluate the antioxidant activities of Se-enriched mycelia in vivo in terms of the values of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and malondialdehyde (MDA). The optimum parameters of Se-enrichment under the optimal Se concentration (150 microg/mL) in media were as follows: biomass 8.11 +/- 0.25 g/L, Se content in mycelia 4,727.68 +/- 13 microg/g, Se-accumulated rate 24.68 +/- 1.67%, and percentage of organic Se 96.27 +/- 3.26%. The mainly subsistent forms of selenium in Se-enriched mycelia were selenoprotein and selenium-polysaccharide. The contents of total amino acids (TAA) and essential amino acids (EAA) in Se-enriched mycelia were increased by 13.5 +/- 1.09% and 12.8 +/- 0.89%, respectively. It was efficient for Se-enriched mycelia to elevate GSH-Px and SOD activities and decrease MDA content. These results indicated that Se-enriched mycelia of S. rugoso-annulata represent a novel dietary source of bioavailable supplemental selenium.
硒（Se）是人和动物必需的微量元素。皱环球盖菇是一种富含多种生物活性成分的营养功能性蘑菇。本研究旨在探讨皱环球盖菇在液体培养中的富硒特性，并根据谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）和丙二醛（MDA）的值评估富硒菌丝体在体内的抗氧化活性。在培养基中最佳硒浓度（150μg/mL）下的最佳富硒参数如下：生物量8.11±±0.25g/L，菌丝体中硒含量4727.68±±13μg/g，硒积累率24.68±±1.67%，有机硒百分比96.27±±3.26%。富硒菌丝体中硒的主要存在形式是硒蛋白和硒多糖。富硒菌丝体中总氨基酸（TAA）和必需氨基酸（EAA）的含量分别增加了13.5±±1.09%和12.8±±0.89%。富硒菌丝体提高GSH-Px和SOD活性并降低MDA含量是有效的。这些结果表明，皱环球盖菇富硒菌丝体是一种新型的可生物利用的补充硒的膳食来源。
