Cui Hua-Li, Liu Zeng-Wu, Fang Yu-Ming, Kai Gui-Qing, Chen Yan
School of Life Science, Anhui University, Hefei 230039, China.
Zhong Yao Cai. 2011 May;34(5):782-6.
To optimize the conditions for exopolysaccharides (EPS) production by Morchella esculenta GIM 5.69.
A Plackett-Burman design was used to evaluate the effects of variables factors. The optimal combined conditions for maximum polysaccharide production were further optimized by response surface methodology.
The optimal fermentation conditions were determined as follows: glucose 30%, cultivation time 5.98 d, rotary speed 217.44 r/min. The average yield of polysaccharide validation experiments was 53.04%, the relative error was 1.64%.
This research has the vital significance regarding the Morchella esculenta polysaccharide function of the product development and the application.
优化羊肚菌GIM 5.69产胞外多糖（EPS）的条件。
采用Plackett-Burman设计评估各变量因素的影响。通过响应面法进一步优化多糖产量最大化的最佳组合条件。
确定最佳发酵条件如下：葡萄糖30%，培养时间5.98天，转速217.44转/分钟。多糖验证实验的平均产量为53.04%，相对误差为1.64%。
本研究对于羊肚菌多糖产品开发及应用具有重要意义。
