Programa de Anatomia, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Cereb Cortex. 2010 Nov;20(11):2583-91. doi: 10.1093/cercor/bhq006. Epub 2010 Feb 12.
The mammalian subventricular zone (SVZ) contains progenitors derived from cerebral cortex radial glia cells, which give rise to glutamatergic pyramidal neurons during embryogenesis. However, during postnatal life, SVZ generates neurons that migrate and differentiate into olfactory bulb γ-aminobutyric acid (GABA)ergic interneurons. In this work, we tested if SVZ cells are able to produce glutamatergic neurons if confronted with the embryonic cortical ventricular zone environment. Different from typical SVZ chain migration, cells from P9-P11 SVZ explants migrate into embryonic cortical slices individually, many of which radially oriented. An average of 82.5% of green fluorescent protein-positive cells were immunolabeled for neuronal marker class III β-tubulin. Invading cells differentiate into multiple morphologies, including a pyramidal-like morphotype. A subset of these cells are GABAergic; however, about 28% of SVZ-derived cells are immunoreactive for glutamate. Adult SVZ explants also give rise to glutamatergic neurons in these conditions. Taken together, our results indicate that SVZ can be a source of glutamatergic cortical neurons when submitted to proper environmental cues.
哺乳动物的侧脑室下区 (SVZ) 包含源自大脑皮层放射状胶质细胞的祖细胞，这些祖细胞在胚胎发生过程中产生谷氨酸能锥体神经元。然而，在出生后，SVZ 产生迁移并分化为嗅球 γ-氨基丁酸 (GABA) 能中间神经元的神经元。在这项工作中，我们测试了 SVZ 细胞在面临胚胎皮质脑室区环境时是否能够产生谷氨酸能神经元。与典型的 SVZ 链迁移不同，来自 P9-P11 SVZ 外植体的细胞单独迁移到胚胎皮质切片中，其中许多呈放射状排列。平均有 82.5% 的绿色荧光蛋白阳性细胞被神经元标志物 III 型 β-微管蛋白免疫标记。入侵细胞分化为多种形态，包括类似锥体的形态。这些细胞中的一部分是 GABA 能的；然而，大约 28% 的 SVZ 衍生细胞对谷氨酸呈免疫反应性。在这些条件下，成年 SVZ 外植体也产生谷氨酸能皮质神经元。总之，我们的结果表明，SVZ 可以成为在适当环境线索作用下产生谷氨酸能皮质神经元的来源。
