Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
College of Agriculture and Natural Resources, Department of Soil & Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Int J Syst Evol Microbiol. 2011 Jan;61(Pt 1):65-70. doi: 10.1099/ijs.0.020545-0. Epub 2010 Feb 12.
A Gram-stain-positive, non-endospore-forming actinobacterium (CC-12301(T)) was isolated from soil attached to a spawn used in the laboratory to grow the edible mushroom Agaricus brasiliensis. Based on 16S rRNA gene sequence similarities, strain CC-12301(T) was shown to belong to the genus Gordonia and was most closely related to the type strains of Gordonia hydrophobica (97.6 % similarity), Gordonia terrae (97.5 %), Gordonia amarae (97.5 %) and Gordonia malaquae (97.4 %). The quinone system was determined to consist predominantly of menaquinone MK-9(H(2)), minor amounts of MK-8(H(2)) and MK-7(H(2)). The polar lipid profile consisted of the major compounds diphosphatidylglycerol and phosphatidylethanolamine, moderate amounts of two phosphatidylinositol mannosides and phosphatidylinositol and minor amounts of phosphatidylglycerol, three unidentified glycolipids, two phosphoglycolipids and a phospholipid. Mycolic acids were present. These chemotaxonomic traits and the major fatty acids, which were C(16 : 1) cis9, C(16 : 0), C(18 : 1) and tuberculostearic acid (10-methyl C(18 : 0)), supported the affiliation of strain CC-12301(T) to the genus Gordonia. The results of physiological and biochemical tests allowed clear phenotypic differentiation of strain CC-12301(T) from the most closely related Gordonia species. Strain CC-12301(T) therefore represents a novel species, for which the name Gordonia humi sp. nov. is proposed, with the type strain CC-12301(T) (=DSM 45298(T) =CCM 7727(T)).
一株革兰氏阳性、非芽孢形成放线菌（CC-12301(T)）从实验室用于种植可食用蘑菇巴西蘑菇（Agaricus brasiliensis）的种菌上附着的土壤中分离得到。根据 16S rRNA 基因序列相似性，菌株 CC-12301(T)被归为戈登氏菌属，与戈登氏嗜旱菌（97.6%相似）、戈登氏壤杆菌（97.5%相似）、戈登氏黄杆菌（97.5%相似）和戈登氏霉臭杆菌（97.4%相似）的模式菌株最为密切相关。醌类系统主要由menaquinone MK-9(H(2))组成，少量 MK-8(H(2))和 MK-7(H(2))。极性脂谱由主要化合物双磷脂酰甘油和磷脂酰乙醇胺、两种磷脂酰肌醇甘露糖苷和磷脂酰肌醇的中等含量、少量磷脂酰甘油、三种未鉴定的糖脂、两种磷酸糖脂和一种磷脂组成。存在分枝菌酸。这些化学分类特征和主要脂肪酸（C16:1 cis9、C16:0、C18:1 和结核硬脂酸（10-甲基 C18:0））支持菌株 CC-12301(T)属于戈登氏菌属。生理生化试验结果使菌株 CC-12301(T)与最密切相关的戈登氏菌属物种明显区别开来。因此，菌株 CC-12301(T)代表一种新的物种，提议命名为戈登氏土栖菌（Gordonia humi）sp. nov.，其模式菌株为 CC-12301(T)（=DSM 45298(T) =CCM 7727(T)）。
