Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e.V., Hans-Knöll-Institut, D-07745 Jena, Germany.
Int J Syst Evol Microbiol. 2011 Jan;61(Pt 1):128-131. doi: 10.1099/ijs.0.022277-0. Epub 2010 Feb 19.
A Gram-stain-positive, non-spore-forming actinobacterium, designated 15-Je-017(T), was isolated from wall material of an indoor environment. The isolate formed a rudimentary substrate mycelium that fragmented into rod-shaped cells. On the basis of 16S rRNA gene sequence analysis, strain 15-Je-017(T) was shown to belong to the genus Jiangella and was most closely related to Jiangella alba YIM 61503(T) (99.7 % 16S rRNA gene sequence similarity), Jiangella alkaliphila D8-87(T) (99.0 %) and Jiangella gansuensis YIM 002(T) (99.0 %). The predominant menaquinone was MK-9(H(4)). Whole-cell hydrolysates contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell wall and rhamnose and glucose as the main sugars. Mycolic acids were absent. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and seven unknown phospholipids. The fatty acid profile contained major amounts (>5 %) of anteiso-C(15 : 0), iso-C(16 : 0), iso-C(15 : 0), iso-C(17 : 0), anteiso-C(17 : 0) and C(17 : 1)ω8c, which supported the affiliation of strain 15-Je-017(T) to the genus Jiangella. DNA-DNA relatedness and physiological and biochemical tests allowed the differentiation of strain 15-Je-017(T) from the type strains of the three known Jiangella species. Strain 15-Je-017(T) represents a novel Jiangella species, for which we propose the name Jiangella muralis sp. nov., with type strain 15-Je-017(T) (=DSM 45357(T) =CCM 7680(T)).
一株革兰氏阳性、非孢子形成的放线菌，命名为 15-Je-017(T)，从室内环境的墙壁材料中分离得到。该分离株形成了原始的基质菌丝，然后分裂成杆状细胞。基于 16S rRNA 基因序列分析，菌株 15-Je-017(T)属于 Jiangella 属，与 Jiangella alba YIM 61503(T)（99.7% 16S rRNA 基因序列相似性）、Jiangella alkaliphila D8-87(T)（99.0%）和 Jiangella gansuensis YIM 002(T)（99.0%）最为密切相关。主要的menaquinone 是 MK-9(H4)。全细胞水解产物中细胞壁的特征二氨基庚二酸是 ll-二氨基庚二酸，主要糖是鼠李糖和葡萄糖。不存在分枝菌酸。极性脂谱由双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和七种未知磷脂组成。脂肪酸谱含有大量（>5%）anteiso-C(15:0)、iso-C(16:0)、iso-C(15:0)、iso-C(17:0)、anteiso-C(17:0)和 C(17:1)ω8c，这支持了菌株 15-Je-017(T)属于 Jiangella 属。DNA-DNA 同源性和生理生化试验允许将菌株 15-Je-017(T)与三种已知 Jiangella 种的模式株区分开来。菌株 15-Je-017(T)代表一种新的 Jiangella 种，我们提议将其命名为 Jiangella muralis sp. nov.，其模式株为 15-Je-017(T)（=DSM 45357(T)=CCM 7680(T)）。
