Division of Medical Microbiology, Department of Clinical and Experimental Medicine, Linköping University, SE-581 85, Linköping, Sweden.
J Water Health. 2010 Jun;8(2):247-56. doi: 10.2166/wh.2009.159. Epub 2009 Nov 9.
Tetracycline-resistant bacteria and genes encoding tetracycline resistance are common in anthropogenic environments. We studied how wastewater treatment affects the prevalence and concentration of two genes, tetA and tetB, that encode resistance to tetracycline. Using real-time polymerase chain reaction (PCR) we analysed wastewater samples collected monthly for one year at eight key-sites in a full-scale municipal wastewater treatment plant (WWTP). We detected tetA and tetB at each sampling site and the concentration of both genes, expressed per wastewater volume or per total-DNA, decreased over the treatment process. The reduction of tetA and tetB was partly the result of the sedimentation process. The ratio of tetA and tetB, respectively, to total DNA was lower in or after the biological processes. Taken together our data show that tetracycline resistance genes occur throughout the WWTP, and that the concentrations are reduced under conventional operational strategies.
耐四环素细菌和编码四环素抗性的基因在人为环境中很常见。我们研究了废水处理如何影响两种基因（tetA 和 tetB）的流行和浓度，这两种基因编码对四环素的抗性。我们使用实时聚合酶链反应（PCR）分析了在一个大型市政废水处理厂（WWTP）的八个关键地点每月收集的废水样本一年。我们在每个采样点检测到 tetA 和 tetB，并且每个废水体积或总 DNA 的表达量都随着处理过程而降低。tetA 和 tetB 的减少部分是由于沉淀过程。在生物过程中或之后，tetA 和 tetB 分别与总 DNA 的比值较低。总的来说，我们的数据表明，四环素抗性基因存在于整个 WWTP 中，并且在常规操作策略下，浓度会降低。
