Lu Lunhui, Liu Jie, Li Zhe, Liu Zhiping, Guo Jinsong, Xiao Yan, Yang Jixiang
CAS Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, China.
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, China.
Front Microbiol. 2018 Aug 17;9:1911. doi: 10.3389/fmicb.2018.01911. eCollection 2018.
The widespread use of antibiotics and the induced antibiotic resistance genes have attracted much attention in recent years. The longshore sediments in the water-level-fluctuating zone of the Three Gorges Reservoir were selected to investigate the spatial-temporal distribution of antibiotics and antibiotic resistance genes in two different operation stages (low-water level in summer and high-water level in winter). Three kinds of tetracycline antibiotics (tetracycline, oxytetracycline, and chlortetracycline) and three kinds of tetracycline resistance genes [(A), (C), and (M)] were analyzed and quantified. The results showed that the distribution of tetracyclines and resistance genes in riverine, transition and lacustrine zones showed a certain regularity, and the tetracycline antibiotics concentration and the total abundance of the tetracycline resistance genes were highest in the transition zone, and then the riverine zone. Meanwhile, there were significant seasonal variations of tetracycline and the resistance genes. The concentrations of the tetracycline and resistance genes were higher in summer than those in winter, while the relative abundance of resistance genes was higher in winter. It was suggested that the different seasonal distribution of antibiotics and resistance genes may be correlated with the reservoir operation in the Three Gorges Reservoir and the higher use of antibiotics in winter. In addition, Pearson correlation analysis showed that the concentrations of the tetracycline, class 1 integron and 16S rRNA were positively correlated with the abundance of the tetracycline resistance genes.
近年来，抗生素的广泛使用及其诱导产生的抗生素抗性基因备受关注。选取三峡水库消落带的长江沿岸沉积物，研究两种不同运行阶段（夏季低水位和冬季高水位）抗生素及抗生素抗性基因的时空分布。分析并定量了三种四环素类抗生素（四环素、土霉素和金霉素）以及三种四环素抗性基因[（A）、（C）和（M）]。结果表明，河流区、过渡区和湖泊区的四环素及抗性基因分布呈现一定规律，四环素类抗生素浓度和四环素抗性基因的总丰度在过渡区最高，其次是河流区。同时，四环素及抗性基因存在显著的季节变化。四环素及抗性基因的浓度夏季高于冬季，而抗性基因的相对丰度冬季较高。研究表明，抗生素和抗性基因的不同季节分布可能与三峡水库的运行以及冬季抗生素使用量较高有关。此外，Pearson相关性分析表明，四环素、1类整合子和16S rRNA的浓度与四环素抗性基因的丰度呈正相关。
