Woody C D, Gruen E, Birt D
Department of Anatomy, UCLA Center for Health Sciences 90024.
Brain Res. 1991 Jan 18;539(1):76-84. doi: 10.1016/0006-8993(91)90688-r.
Single electrode voltage clamp recordings were made during Pavlovian conditioning of single units of the motor cortex of cats. Units that developed a conditioned spike discharge in response to a click conditioned stimulus (CS) after pairing the click with glabella tap and local ionophoretic application of glutamate showed increases in input resistance and reductions of an early outward current induced by depolarizing commands and by return to holding potentials after hyperpolarizing commands. Changes in later currents were also found in some cells. Units that failed to develop a conditioned response did not show these changes. The decreases in membrane currents could contribute to an increased spike discharge in response to the CS as could the increased input resistance observed after conditioning. Conductance changes of this type may serve as engrams by which some forms of memory and learning are expressed across both vertebrate and invertebrate species.
在对猫运动皮层单个神经元进行巴甫洛夫条件反射实验期间，进行了单电极电压钳记录。在将点击声与眉间轻叩以及局部离子电泳施加谷氨酸配对后，对点击条件刺激（CS）产生条件性尖峰放电的神经元，其输入电阻增加，并且由去极化指令以及超极化指令后回到静息电位所诱发的早期外向电流减小。在一些细胞中还发现了后期电流的变化。未能产生条件反应的神经元未表现出这些变化。膜电流的减小可能导致对CS的尖峰放电增加，条件反射后观察到的输入电阻增加也可能有此作用。这种类型的电导变化可能作为记忆痕迹，通过它在脊椎动物和无脊椎动物物种中表达某些形式的记忆和学习。
