Department of Biology and Medical Parasitology, Poznań University of Medical Sciences, 10 Fredry Street, 61-701, Poznań, Poland.
Parasitol Res. 2010 Apr;106(5):1015-9. doi: 10.1007/s00436-010-1766-5. Epub 2010 Feb 13.
Giardia duodenalis (syn. G. intestinalis, G. lamblia) is an intestinal protozoan parasite that infects humans and a wide range of mammals that includes dogs. Highly significant genetic heterogeneity has been found within this species, while only genotypes from assemblages A and B have zoonotic potential. Although Giardia infection in dogs has been reported worldwide, there is increasing molecular evidence that dogs may be infected with host-specific genotypes (C and D) as well as zoonotic ones (A and B). Therefore, dogs play a role as a potential source of Giardia infection impacting humans and other Canidae. Fecal samples from privately owned dogs and from dogs kept in two shelters in the west-central region of Poland were examined using a microscope and polymerase chain reaction. The total prevalence of Giardia in specimens was 1.9%. Deoxyribonucleic acid was extracted only from two out of the three Giardia-positive fecal samples. After amplification using the G7 and G759 beta-giardin primers, 753 bp amplicons were obtained, and both amplification products were sequenced. Sequence and phylogenetic analyses showed that both G. duodenalis isolates were dog-specific genotypes (C and D).
十二指肠贾第鞭毛虫（又称 G. 肠内，G. 蓝氏贾第鞭毛虫）是一种肠道原生动物寄生虫，感染人类和包括狗在内的广泛哺乳动物。在该物种内发现了高度显著的遗传异质性，而只有来自 A 组和 B 组的基因型具有人畜共患潜力。尽管狗中的贾第鞭毛虫感染已在全球范围内得到报道，但越来越多的分子证据表明，狗可能感染有宿主特异性基因型（C 和 D）以及人畜共患基因型（A 和 B）。因此，狗在影响人类和其他犬科动物的贾第鞭毛虫感染中发挥了潜在的传染源作用。使用显微镜和聚合酶链反应检查了来自波兰中西部地区两家收容所的私人拥有的狗和狗的粪便样本。在标本中，贾第鞭毛虫的总流行率为 1.9%。仅从三个贾第鞭毛虫阳性粪便样本中的两个中提取脱氧核糖核酸。使用 G7 和 G759 β-贾第鞭毛虫引物扩增后，获得了 753 bp 扩增子，并且对两个扩增产物进行了测序。序列和系统发育分析表明，两种 G. 十二指肠虫均为狗特异性基因型（C 和 D）。
