Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Parasitol Res. 2012 Dec;111(6):2419-22. doi: 10.1007/s00436-012-3100-x. Epub 2012 Sep 15.
The prevalence of Giardia duodenalis was determinate in faecal samples from dogs and cats in Madrid, Spain and molecular characterisation of isolates. A total of 604 and 144 faecal samples from dogs and cats, respectively, were analysed by routine coprological methods. The prevalence of G. duodenalis was 16.4 % (99/604) in dogs and 4.2 % (6/144) in cats. Sixty-four G. duodenalis isolates (63 from dogs and 1 from a cat) were characterised using glutamate dehydrogenase and β-giardin genes by PCR-RFLP. The single cat sample showed a mixed infection by assemblages A + F. The assemblages found in the dog samples were A, B, C, D and E, both as single and as mixed infections. The zoonotic assemblages A and B were found in 56 (88.8 %) G. duodenalis-positive samples with 15.9 % of samples having assemblage A (10/63) and 73 % of samples with assemblage B (46/63), indicating high potential zoonotic risk and public health significance.
在西班牙马德里的犬猫粪便样本中确定了十二指肠贾第虫的流行情况，并对分离株进行了分子特征分析。分别用常规粪便检查方法分析了 604 份犬和 144 份猫的粪便样本。犬的十二指肠贾第虫感染率为 16.4%（99/604），猫的感染率为 4.2%（6/144）。对 64 株（63 株来自犬，1 株来自猫）十二指肠贾第虫分离株进行了谷氨酸脱氢酶和β-微管蛋白基因 PCR-RFLP 分析。唯一的猫样本显示出混合感染了 A+F 组合。在犬样本中发现的组合有 A、B、C、D 和 E，既有单一感染也有混合感染。发现了具有潜在人畜共患风险的 A 和 B 组合，63 份阳性样本中有 56 份（88.8%）为 A 和 B 组合，其中 15.9%的样本为 A 组合（10/63），73%的样本为 B 组合（46/63），表明具有较高的人畜共患风险和公共卫生意义。
