鉴定单羧酸转运蛋白 1（MCT1）与 Basigin 基因产物和 L1cam 的结合亲和力。
Characterization of monocarboxylate transporter 1 (MCT1) binding affinity for Basigin gene products and L1cam.
机构信息
Department of Biology, University of North Florida, 1 UNF Drive, Jacksonville, FL 32224, USA.
出版信息
Cell Mol Neurobiol. 2010 Jul;30(5):671-4. doi: 10.1007/s10571-010-9503-2. Epub 2010 Feb 13.
The purpose of this study was to determine the binding affinities of Basigin gene products and neural cell adhesion molecule L1cam for monocarboxylate transporter-1 (MCT1). ELISA binding assays were performed in which recombinant proteins of the transmembrane domains of Basigin gene products and L1cam were incubated with MCT1 captured from mouse brain. It was determined that Basigin gene products bind MCT1 with moderate affinity, but L1cam does not bind MCT1. Despite a high degree of sequence conservation between Basigin gene products and L1cam, the sequences are different enough to prevent L1cam from interacting with MCT1.
本研究旨在确定 Basigin 基因产物和神经细胞粘附分子 L1cam 与单羧酸转运蛋白-1（MCT1）的结合亲和力。进行了 ELISA 结合分析，其中将 Basigin 基因产物和 L1cam 的跨膜结构域的重组蛋白与从小鼠脑中捕获的 MCT1 孵育。结果表明，Basigin 基因产物与 MCT1 具有中等亲和力结合，但 L1cam 不与 MCT1 结合。尽管 Basigin 基因产物和 L1cam 之间具有高度的序列保守性，但序列差异足以防止 L1cam 与 MCT1 相互作用。
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