固定化葡萄糖氧化酶的性质及其酶活的增强。
Properties of immobilized glucose oxidase and enhancement of enzyme activity.
机构信息
Gazi University, Faculty of Science, Chem. Dpt., Ankara, Turkey.
出版信息
Artif Cells Nanomed Biotechnol. 2013 Aug;41(4):264-8. doi: 10.3109/10731199.2012.731415. Epub 2012 Oct 30.
In this study; glucose oxidase (GOD) and MnO2 were immobilized in alginate gel beads using the entrapment method and effects of oxygen vectors on immobilized GOD activity were investigated. The specific activity of free enzyme was 1.3 units/mg protein whereas that of immobilized enzyme was 8.4 × 10(-1) units/mg protein. The Km values for free and immobilized enzyme were found to be 2.7 mM, 2.9 mM; Vmax values 3.64 × 10(-1) μmol/min.mL, 2.61 × 10(-1) μmol/min.mL, respectively. When 4% (v/v) silicone oil or soybean oil was used, the activity of immobilized GOD increased 1.7 times and 1.3 times, respectively, compared to media without these oils.
在这项研究中，葡萄糖氧化酶（GOD）和 MnO2 采用包埋法固定在海藻酸钠凝胶珠中，并研究了氧载体对固定化 GOD 活性的影响。游离酶的比活为 1.3 单位/毫克蛋白，而固定化酶的比活为 8.4×10(-1)单位/毫克蛋白。游离酶和固定化酶的 Km 值分别为 2.7mM 和 2.9mM；Vmax 值分别为 3.64×10(-1)μmol/min.mL 和 2.61×10(-1)μmol/min.mL。当使用 4%（v/v）硅油或豆油时，与不含这些油的介质相比，固定化 GOD 的活性分别增加了 1.7 倍和 1.3 倍。
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