Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Cordoba, Argentina.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2010 Mar;27(3):373-9. doi: 10.1080/19440040903403362.
The aim was to carry out a survey of aflatoxin M(1) (AFM(1)) in raw whole milk from bulk tanks. The sample collection was performed in farms located in one the most important milk-production zones in the centre of Argentina. A total of 94 samples of milk from 47 dairy farms were analysed. AFM(1) analysis involved the use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) with prior purification of the extracts using immunoaffinity columns. AFM(1) incidence in raw milk was high as 63.8% and levels were between not detected to 0.07 microg l(-1). Several contaminated samples (39%) were over the European Commission limit for infant milk (0.025 microg l(-1)), although none of samples were above Argentine legislation. Estimates of AFM(1) intake were assessed for different age populations. The average AFM(1) estimated daily intakes were 1.6, 0.5, 0.17 ng kg(-1) body weight day(-1) for 4-year-old babies, young children, and adults, respectively. All tested farms used pastures and silages at similar composition. Even though some farms (13) employed high-risk supplementary feeds, such as peanut pod and/or cotton seed, no statistically significant differences were observed between groups. Information from AFM(1) levels in milk in Argentina is limited. A systematic AFM(1) monitoring programme must be performed by means of accurate and reliable analytical techniques as a strategy for protecting milk consumers.
本研究旨在对来自散装奶罐的生牛乳中的黄曲霉毒素 M1（AFM1）进行调查。样本采集在阿根廷中部一个最重要的牛奶生产区的农场进行。共分析了来自 47 个奶牛场的 94 份牛奶样本。AFM1 的分析采用液相色谱-串联质谱法（LC-MS/MS），并使用免疫亲和柱对提取物进行预先纯化。生牛乳中 AFM1 的检出率很高，达 63.8%，浓度范围为未检出至 0.07μg/L。有几个污染样本（39%）超过了欧盟婴儿配方奶的限量（0.025μg/L），尽管没有一个样本超过阿根廷的法规。还评估了不同年龄人群对 AFM1 的摄入量。4 岁婴儿、幼儿和成年人的平均 AFM1 估计日摄入量分别为 1.6、0.5 和 0.17ng/kg 体重/天。所有测试的农场都使用相似组成的牧场和青贮饲料。尽管一些农场（13 家）使用了高风险的补充饲料，如花生荚和/或棉籽，但组间未观察到统计学上的显著差异。关于阿根廷牛奶中 AFM1 水平的信息有限。必须通过准确可靠的分析技术实施系统的 AFM1 监测计划，作为保护牛奶消费者的策略。
