Department of Pathology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario - Canada.
Int J Biol Markers. 2010 Jan-Mar;25(1):32-7. doi: 10.1177/172460081002500105.
To analyze the expression of human kallikrein 14 (KLK14) in salivary gland tumors.
A standard immunoperoxidase staining technique was used to assess the expression profile of KLK14 in normal salivary glands and tumors including pleomorphic adenoma (PA; n=17), adenoid cystic carcinoma (ACC; n=13) and mucoepidermoid carcinoma (MEC; n=9). Tumor stage, grade, patient age and gender, and site of occurrence were recorded. These clinical parameters were correlated with KLK14 levels in malignant tumors. The expression profiles for KLK3, 5, 6, 8 and 13 were also retrieved.
Normal salivary glands, PA, ACC and MEC showed strong expression of KLK14 in ductal and non-ductal cells. Both PA and ACC showed higher KLK14 levels than normal glands and MEC tissues. There were no statistically significant associations between levels of KLK14 and clinical parameters.
The differences in the levels of KLK14 suggest that KLKs may aid in the differential diagnosis of salivary gland tumors. The coexpression of KLKs suggests their possible involvement in an enzymatic pathway activated in salivary gland. KLK14 may be a promising new biomarker in salivary gland tumors.
分析人激肽释放酶 14（KLK14）在唾液腺肿瘤中的表达。
采用标准免疫过氧化物酶染色技术评估 KLK14 在正常唾液腺和肿瘤中的表达谱，包括多形性腺瘤（PA；n=17）、腺样囊性癌（ACC；n=13）和黏液表皮样癌（MEC；n=9）。记录肿瘤分期、分级、患者年龄和性别以及发病部位。将这些临床参数与恶性肿瘤中的 KLK14 水平相关联。还检索了 KLK3、5、6、8 和 13 的表达谱。
正常唾液腺、PA、ACC 和 MEC 在导管细胞和非导管细胞中均表现出 KLK14 的强表达。PA 和 ACC 的 KLK14 水平均高于正常腺体和 MEC 组织。KLK14 水平与临床参数之间无统计学显著相关性。
KLK14 水平的差异表明 KLKs 可能有助于唾液腺肿瘤的鉴别诊断。KLKs 的共表达表明它们可能参与了唾液腺中激活的酶促途径。KLK14 可能是唾液腺肿瘤有前途的新生物标志物。
