Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.
Luminescence. 2011 Mar-Apr;26(2):118-24. doi: 10.1002/bio.1193.
A novel phenomenon of dual chemiluminescence (CL) was observed for the KIO4-luminol-Mn(2+) system in strong alkaline solutions using the stopped-flow technique. Scavenging study of the reactive oxygen species (ROS) suggested that the two CL peaks originated from different CL pathways precipated by distinct ROS (O2(-) and •OH for the first peak, mainly 1O2 for the second peak). Generation of these ROS at different time intervals from the reactions involving IO4(-), O2, and Mn(2+) and their subsequent reactions with luminol induced the intense CL emission. The relative intensity of the two CL peaks can be tuned over a wide range by varying the concentrations of Mn(2-), luminol and KIO4. Because of the involvement of different ROS in each pathway, the two CL peaks could respond quite differently to various substances. Moreover, variation of the intensity ratio of the two CL peaks altered the relative proportions of the corresponding ROS, thereby changing their responses to a given substance. The dual CL emission acts like a pair of tunable probes and it is believed that this CL system has great potential in analytical applications.
在强碱性溶液中，利用停流技术观察到 KIO4-鲁米诺-Mn(2+)体系的一种新的双化学发光（CL）现象。对活性氧（ROS）的清除研究表明，两个 CL 峰源于不同的 CL 途径，由不同的 ROS（O2(-)和•OH 为第一个峰，主要为 1O2 为第二个峰）沉淀。这些 ROS 是由涉及 IO4(-)、O2 和 Mn(2+)的反应在不同时间间隔产生的，并且它们随后与鲁米诺反应诱导强烈的 CL 发射。通过改变 Mn(2+)、鲁米诺和 KIO4 的浓度，可以在很宽的范围内调节两个 CL 峰的相对强度。由于每个途径中涉及不同的 ROS，两个 CL 峰对各种物质的响应可能非常不同。此外，两个 CL 峰的强度比的变化改变了相应的 ROS 的相对比例，从而改变了它们对给定物质的响应。双 CL 发射类似于一对可调谐的探针，并且相信该 CL 系统在分析应用中具有很大的潜力。
