Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Luminescence. 2010 Jan-Feb;25(1):43-9. doi: 10.1002/bio.1141.
Chemiluminescence (CL) from the oxidation of luminol with potassium periodate in strong alkaline solutions was greatly enhanced by the combined effect of gallic acid, acetaldehyde and Mn(2+). The CL spectra exhibited only one emission band at 425 nm, indicating 3-aminophthalate as the emitting species. Various scavengers for superoxide anion, hydroxyl radical and singlet oxygen quenched the CL emission very efficiently (74-100%), suggesting the possible involvement of these reactive oxygen species (ROS) in the CL reactions. It is postulated that oxidation of gallic acid and acetaldehyde by periodate catalyzed by Mn(2+) generates these ROS, which then react with luminol to enhance the CL emission. We also found that the enhanced CL emission was strongly inhibited by catecholamines, probably because of their effective scavenging of ROS. Based on this observation, a simple, rapid and sensitive new CL method was developed for the determination of catecholamines. The detection limits (3sigma) for dopamine, l-dopa, norepinephrine and epinephrine were 0.63, 1.37, 0.56 and 14.3 nmol/L, respectively. The linear range was 1-10 nmol/L; relative standard deviations were 0.71-1.34% for 0.1 micromol/mL catecholamines. This CL method was applied to the determination of catecholamines in pharmaceutical injections with satisfactory results.
在强碱性溶液中，过碘酸钾氧化鲁米诺产生的化学发光（CL）通过没食子酸、乙醛和 Mn（2+）的协同作用得到了极大增强。CL 光谱仅在 425nm 处显示出一个发射带，表明 3-氨基邻苯二甲酸盐是发射物质。超氧阴离子、羟基自由基和单线态氧的各种清除剂非常有效地猝灭 CL 发射（74-100%），表明这些活性氧物质（ROS）可能参与了 CL 反应。据推测，过碘酸盐催化 Mn（2+）氧化没食子酸和乙醛生成这些 ROS，然后与鲁米诺反应增强 CL 发射。我们还发现，儿茶酚胺强烈抑制增强的 CL 发射，可能是因为它们有效地清除了 ROS。基于这一观察结果，开发了一种简单、快速和灵敏的新 CL 方法来测定儿茶酚胺。多巴胺、左旋多巴、去甲肾上腺素和肾上腺素的检测限（3sigma）分别为 0.63、1.37、0.56 和 14.3nmol/L。线性范围为 1-10nmol/L；对于 0.1μmol/mL 儿茶酚胺，相对标准偏差为 0.71-1.34%。该 CL 方法已应用于药物注射剂中儿茶酚胺的测定，结果令人满意。
