Key Laboratory of Luminescence and Real-time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
Luminescence. 2012 May-Jun;27(3):217-22. doi: 10.1002/bio.1335. Epub 2011 Jul 20.
A post-chemiluminescence (PCL) phenomenon was observed when chloramphenicol was injected into a mixture of luminol and potassium periodate after the chemiluminescence (CL) reaction of luminol-potassium periodate had finished. The possible reaction mechanism was proposed based on studies of the CL kinetic characteristics, the CL spectra, the fluorescence spectra and the UV-vis absorption spectra of the related substances. Based on the PCL reaction, a rapid and sensitive method for the determination of chloramphenicol was established. The linear response range was 6.0 × 10(-7) -1.0 × 10(-5) mol/L, with a correlation coefficient of 0.9986. The relative standard deviation (RSD) for 5.0 × 10(-6) mol/L chloramphenicol was 2.3% (n = 11). The detection limit was 1.6 × 10(-7) mol/L. The method has been applied to the determination of chloramphenicol in pharmaceutical samples with satisfactory results.
氯霉素在鲁米诺-高碘酸钾化学发光反应结束后注入到鲁米诺和高碘酸钾的混合物中时，观察到化学发光（CL）后发光（PCL）现象。根据 CL 动力学特性、CL 光谱、荧光光谱和相关物质的紫外可见吸收光谱的研究，提出了可能的反应机制。基于 PCL 反应，建立了一种快速灵敏的测定氯霉素的方法。线性响应范围为 6.0×10(-7)-1.0×10(-5)mol/L，相关系数为 0.9986。对于 5.0×10(-6)mol/L 氯霉素，相对标准偏差（RSD）为 2.3%（n=11）。检测限为 1.6×10(-7)mol/L。该方法已应用于药物样品中氯霉素的测定，结果令人满意。
