Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
Cell Biol Int. 2010 Apr 8;34(5):531-6. doi: 10.1042/CBI20090498.
Cytokines such as VEGF (vascular endothelial growth factor) and ILs (interleukins) are involved in follicular development in the mammalian ovary. The aim of the present study is to examine the transcripts of IL-8 and -1 that differ during follicular development; the relationships between IL-8, IL-1 and VEGF in theca cells is still unknown. We first examined the gene expression of IL-8, IL-1beta and their respective receptors, CXCR1 and IL-1R1 in the theca cells of PRF (preselection) and POF (postselection follicles) from the bovine ovary. Expression of IL-8 and CXCR1 genes were observed in POF, whereas expression of IL-1beta and IL-1R1 genes was observed in both follicles. Secondly, we examined the effects of VEGF on the expression of IL-8, IL-1beta and their receptors genes in cultured bovine theca cells. mRNA expression was quantified by using real-time PCR methods. VEGF stimulates the expression of IL-8 and CXCR1 mRNA. However, VEGF down-regulates the expression of CXCR2 mRNA during the culture period. Expression of IL-1beta and -1R1 mRNA was induced in the cultured theca cells at 48 h. Our data demonstrate that VEGF stimulated the expression of the IL-8 and CXCR1 genes and that CXCR2 expression was suppressed by VEGF, suggesting a follicle stage-dependent expression pattern for the IL-8 system. Furthermore, our results suggest that the transcription system for CXCR genes may have different pathways of VEGF stimulation in bovine theca cells. Taken together, our data suggested that VEGF is associated with the IL system in theca cells in bovine ovary.
细胞因子如 VEGF（血管内皮生长因子）和 ILs（白细胞介素）参与哺乳动物卵巢中的卵泡发育。本研究旨在研究在卵泡发育过程中不同的 IL-8 和 -1 转录本；IL-8、IL-1 和 VEGF 在卵巢的颗粒细胞中的关系尚不清楚。我们首先检查了 PRF（预选）和 POF（后选卵泡）牛卵巢中的颗粒细胞中 IL-8、IL-1β及其各自受体 CXCR1 和 IL-1R1 的基因表达。在 POF 中观察到 IL-8 和 CXCR1 基因的表达，而在两个卵泡中都观察到 IL-1β和 IL-1R1 基因的表达。其次，我们检查了 VEGF 对培养的牛颗粒细胞中 IL-8、IL-1β及其受体基因表达的影响。使用实时 PCR 方法定量 mRNA 表达。VEGF 刺激 IL-8 和 CXCR1 mRNA 的表达。然而，在培养期间，VEGF 下调 CXCR2 mRNA 的表达。在培养的颗粒细胞中，IL-1β和 -1R1 mRNA 在 48 小时被诱导。我们的数据表明，VEGF 刺激了 IL-8 和 CXCR1 基因的表达，并且 VEGF 抑制了 CXCR2 表达，提示 IL-8 系统具有卵泡阶段依赖性表达模式。此外，我们的结果表明，CXCR 基因的转录系统在牛颗粒细胞中可能具有不同的 VEGF 刺激途径。总之，我们的数据表明，VEGF 与牛卵巢颗粒细胞中的 IL 系统有关。
