Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Berne, Freiburgstrasse 7, Berne, Switzerland.
Clin Implant Dent Relat Res. 2011 Mar;13(1):1-12. doi: 10.1111/j.1708-8208.2009.00266.x.
Platelet rich plasma (PRP) has been proposed to be a useful adjunct to bone grafting.
The aim of the present study was to assess new bone formation in bone regeneration procedures using platelet rich plasma (PRP) alone or in combination with autogenous bone.
Four surgically created, monocortical defects 5 mm in diameter in the calvariae of 15 New Zealand rabbits were grafted with a coagulum-filled control, PRP, particulated autogenous bone alone (A), or combined with PRP (A-PRP).
Mean platelet concentration of 1,761,930 ± 680,200/µl was achieved (5.30 ± 2.63 × fold of baseline). Animals were sacrificed 1, 2, and 4 weeks later. Histomorphometric analysis showed no statistical difference for total new bone formation at any time point, however, a detailed analysis revealed a statistically significant higher percentage of lamellar bone than woven bone for the autogenous bone group at 2 weeks; all other groups demonstrated equal percentages of either bone type. At 4 weeks, all groups revealed a statistically greater component of lamellar bone over woven bone. Graft resorption rate was similar for both A and A-PRP. PRP platelet concentration was significantly positively correlated with TGF-beta1 but not with PDGF-AB.
Within the limits of the chosen animal model, this study demonstrated that PRP during early healing, whether alone or mixed with autogenous bone, did not lead to greater bone remodelling, as compared to coagulum. In contrast, autogenous bone alone demonstrated accelerated bone remodelling at 2 weeks.
富血小板血浆（PRP）已被提议作为骨移植的有用辅助手段。
本研究旨在评估单独使用或与自体骨联合使用富含血小板的血浆（PRP）在骨再生过程中的新骨形成。
在 15 只新西兰兔的颅骨中，共创建了 4 个直径为 5mm 的单皮质缺损，并分别用充满凝血块的对照物、PRP、单独的颗粒状自体骨（A）或与 PRP 联合（A-PRP）进行移植。
平均血小板浓度为 1,761,930±680,200/µl（基线的 5.30±2.63 倍）。动物在 1、2 和 4 周后被处死。组织形态计量学分析显示，在任何时间点，总新骨形成均无统计学差异，但详细分析显示，在 2 周时，自体骨组的板层骨比编织骨具有统计学上更高的百分比；所有其他组均显示出相等比例的任何一种骨类型。在 4 周时，所有组均显示出统计学上更多的板层骨成分。A 和 A-PRP 的移植物吸收率相似。PRP 血小板浓度与 TGF-β1 呈显著正相关，但与 PDGF-AB 无关。
在所选动物模型的范围内，本研究表明，与凝血块相比，PRP 在早期愈合时，无论是单独使用还是与自体骨混合使用，都不会导致更大的骨重塑。相比之下，单独使用自体骨在 2 周时表现出更快的骨重塑。
