富血小板血浆治疗糖尿病大鼠颅骨临界尺寸骨缺损的愈合：组织学和组织计量学研究
Bone healing in critical-size defects treated with platelet-rich plasma: a histologic and histometric study in the calvaria of diabetic rat.
作者信息
Mariano Ronaldo, Messora Michel, de Morais Adriana, Nagata Maria, Furlaneto Flávia, Avelino Cássia, Paula Fernanda, Ferreira Sabrina, Pinheiro Marcelo, de Sene João Paulo
机构信息
Division of Bucco-Maxillo-Facial Surgery and Traumatology, Department of Clinic and Surgery, Dental School, Federal University of Alfenas - UNIFAL, Alfenas, Brazil.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Jan;109(1):72-8. doi: 10.1016/j.tripleo.2009.08.003. Epub 2009 Nov 17.
OBJECTIVE
The objective of this study was to histologically analyze the influence of the platelet-rich plasma (PRP) on bone healing in surgically created critical-size defects (CSD) in the calvaria of diabetic rats.
STUDY DESIGN
A total of 20 diabetic rats were divided into 2 groups: C (control) and PRP. A 5-mm diameter CSD was created in the calvarium of each animal. In Group C, the defect was filled by blood clot only. In Group PRP, 0.35 mL of PRP was placed in the defects. All animals were humanely killed 30 days postoperatively. Histometric and histologic analyses were performed. Data were statistically analyzed (t test, P < .05).
RESULTS
No defect completely regenerated with bone. Group PRP had a statistically greater amount of bone formation than Group C (37.22% +/- 6.00% and 21.68% +/- 11.35%, respectively).
CONCLUSION
PRP placed in the defects significantly enhanced bone healing in CSD in the calvaria of diabetic rats both qualitatively and quantitatively.
目的
本研究的目的是从组织学角度分析富血小板血浆（PRP）对糖尿病大鼠颅骨手术造成的临界大小骨缺损（CSD）愈合的影响。
研究设计
总共20只糖尿病大鼠被分为2组：C组（对照组）和PRP组。在每只动物的颅骨上制造一个直径5毫米的临界大小骨缺损。在C组中，缺损仅用血凝块填充。在PRP组中，将0.35毫升PRP置于缺损处。所有动物在术后30天被人道处死。进行了组织计量学和组织学分析。数据进行了统计学分析（t检验，P < 0.05）。
结果
没有缺损完全再生出骨组织。PRP组的骨形成量在统计学上显著高于C组（分别为37.22% +/- 6.00%和21.68% +/- 11.35%）。
结论
置于缺损处的PRP在质量和数量上均显著促进了糖尿病大鼠颅骨临界大小骨缺损的骨愈合。
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