Xie Huanxin, Cao Lei, Ye Linlin, Du Jubao, Shan Guixiang, Hu Jie, Jiang Chunjing, Song Weiqun
Department of Rehabilitation, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China.
Exp Ther Med. 2020 Dec;20(6):279. doi: 10.3892/etm.2020.9409. Epub 2020 Oct 27.
Long-term bone defects are a key clinical problem. Autogenous bone graft remains the gold standard for the treatment of these defects; however, improving the osteogenic properties and reducing the amount of autogenous bone is challenging. Autologous platelet-rich plasma (PRP) has been widely considered for treatment, due to its potentially beneficial effect on bone regeneration and vascularization. The aim of the present study was to explore the effects of autogenous bone particles combined with PRP on repairing segmental bone defects in rabbits. Briefly, a critical-size diaphyseal radius defect was established in 45 New Zealand White rabbits. Animals were randomly divided into four groups, according to the different implants: Group A, empty bone defect; group B, PRP; group C, autogenous bone particles + bone mesenchymal stem cells (BMSCs) on the left radius; group D, autogenous bone particles + PRP + BMSCs on the right radius. Bone samples were collected and further analyzed using X-ray, histology and histomorphometry 4, 8 and 12 weeks post-surgery. In addition, the effect of PRP on cell proliferation was detected by Cell Counting Kit-8 and the concentrations of growth factors (GFs), transforming GF (TGF)-β1 and platelet-derived GF (PDGF), in PRP were verified by ELISA. X-ray, histology and histomorphometry data revealed that the fraction area of the newly formed bone was larger in group D. In addition, PRP could improve cell proliferation, osteogenic differentiation and the release of GFs, TGF-β1 and PDGF-AB. In conclusion, these findings indicated that an autogenous bone particle + PRP + BMSC scaffold may be used as a potential treatment strategy for segmental defects in humans.
长期骨缺损是一个关键的临床问题。自体骨移植仍然是治疗这些缺损的金标准；然而，改善成骨特性并减少自体骨用量具有挑战性。自体富血小板血浆（PRP）因其对骨再生和血管生成可能具有的有益作用而被广泛考虑用于治疗。本研究的目的是探讨自体骨颗粒联合PRP对兔节段性骨缺损修复的影响。简要地说，在45只新西兰白兔中建立临界尺寸的骨干桡骨缺损。根据不同的植入物将动物随机分为四组：A组，空骨缺损；B组，PRP；C组，左侧桡骨自体骨颗粒 + 骨间充质干细胞（BMSCs）；D组，右侧桡骨自体骨颗粒 + PRP + BMSCs。在术后4、8和12周收集骨样本并使用X射线、组织学和组织形态计量学进行进一步分析。此外，通过细胞计数试剂盒-8检测PRP对细胞增殖的影响，并通过酶联免疫吸附测定法（ELISA）验证PRP中生长因子（GFs）、转化生长因子（TGF）-β1和血小板衍生生长因子（PDGF）的浓度。X射线、组织学和组织形态计量学数据显示，D组新形成骨的面积分数更大。此外，PRP可改善细胞增殖、成骨分化以及GFs、TGF-β1和PDGF-AB的释放。总之，这些发现表明自体骨颗粒 + PRP + BMSC支架可能作为人类节段性缺损的一种潜在治疗策略。
