Davey C L, Thakkar H, Newman D J, Price C P
Department of Clinical Biochemistry, St. Bartholomew's and the Royal London School of Medicine and Dentistry, England.
Ther Drug Monit. 1997 Feb;19(1):108-11. doi: 10.1097/00007691-199702000-00021.
We have adapted a latex particle-enhanced immunoassay for serum digoxin to a centrifugal analyser. A 4-microliter serum sample (without pretreatment) inhibits the monoclonal antibody induced aggregation of digoxin-coated latex particles. The total assay time is 10 min and mean analytical recoveries were 98.1%. Intra and interassay precision were < 4.2 and < 15.0%, respectively. Method comparison with an established fluorescence polarisation immunoassay (FPIA) gave R = 0.97, and a Deming regression analysis of particle-enhanced turbidimetric inhibition immunoassay (PETINIA) = FPIA x 0.78 + 0.007 microgram/L (n = 91). There was no evidence of significant interference from digoxin-like immunoreactive compounds in patients with chronic renal failure. This assay can be adapted to most photometric analysers used in routine laboratories and is a significant advance in the sensitivity of latex particle-enhanced immunoassays in a serum matrix.
我们已将用于血清地高辛检测的乳胶颗粒增强免疫测定法适配于离心分析仪。4微升血清样本（无需预处理）可抑制单克隆抗体诱导的地高辛包被乳胶颗粒聚集。总检测时间为10分钟，平均分析回收率为98.1%。批内和批间精密度分别<4.2%和<15.0%。与既定的荧光偏振免疫测定法（FPIA）进行方法比较，得到R = 0.97，颗粒增强比浊抑制免疫测定法（PETINIA）的Deming回归分析结果为PETINIA = FPIA×0.78 + 0.007微克/升（n = 91）。慢性肾衰竭患者中未发现地高辛样免疫反应性化合物产生显著干扰的证据。该检测方法可适配于常规实验室使用的大多数光度分析仪，是血清基质中乳胶颗粒增强免疫测定法灵敏度的一项重大进展。
