Department of Lung Diseases and Tuberculosis, Medical University of Bialystok, Bialystok, Poland.
Eur J Med Res. 2009 Dec 7;14 Suppl 4(Suppl 4):165-9. doi: 10.1186/2047-783x-14-s4-165.
It has recently been described that circulatory and BAL regulatory T-cells (Tregs), defined as CD4+CD25highCD127low are increased in patients with active sarcoidosis compared with other interstitial lung diseases.
We studied prospectively 17 patients (10 women, 7 men) of median age 39 years (range 27-65) with active granulomatous lung diseases (GLD) (10 patients with sarcoidosis (BBS), and 7 with hypersensitivity pneumonitis (HP), and 9 healthy controls. Bronchoalveolar lavage fluid (BAL) and induced sputum Treg counts, CD4+, CD8+, CD25+ cells were quantified by flow cytometry. Disease activity was measured by ACE serum level. Pulmonary function tests were performed using an Elite DL Medgraphics body box.
We found Treg cells count significantly elevated in induced sputum from active GLD (38.3% vs. 7.1% and 5.3% in BBS, HP, and control, respectively). A significantly higher percentage of Treg cells characterized BAL cells from HP patients (2.27%; 9.5%; 2.1%, in BBS, HP and control, respectively). There was a strong correlation with ACE serum level and Treg cell count in BAL fluid of BBS patients, with no such correlation within HP patient group, nor Treg cell count and pulmonary function tests.
Our data suggest a potential role of CD4+CD25highCD127low induced sputum and BAL lymphocytes from patients with active granulomatous lung diseases and hypersensitivity pneumonitis. An increased number of Treg cells in active GLD may be involved in immune regulation in active granulomatous lung diseases. The results indicate that analysis of these cells could be useful as markers of disease activity in granulomatous lung diseases.
最近有人描述，与其他间质性肺疾病相比，活动期结节病患者的循环和 BAL 调节性 T 细胞（Tregs），定义为 CD4+CD25highCD127low，数量增加。
我们前瞻性研究了 17 名（10 名女性，7 名男性）中位年龄 39 岁（范围 27-65 岁）的活动期肉芽肿性肺部疾病（GLD）患者（10 名结节病患者（BBS），7 名过敏性肺炎（HP）患者，9 名健康对照者。通过流式细胞术定量测定支气管肺泡灌洗液（BAL）和诱导痰 Treg 计数、CD4+、CD8+、CD25+细胞。采用 ACE 血清水平测量疾病活动度。使用 Elite DL Medgraphics 体盒进行肺功能测试。
我们发现，来自活动期 GLD 的诱导痰中 Treg 细胞计数显著升高（38.3% vs. BBS、HP 和对照组中的 7.1%和 5.3%）。HP 患者 BAL 细胞中 Treg 细胞的比例明显更高（2.27%；9.5%；2.1%，分别为 BBS、HP 和对照组）。BBS 患者 BAL 液中 ACE 血清水平与 Treg 细胞计数之间存在很强的相关性，而在 HP 患者组中则无相关性，Treg 细胞计数与肺功能检查也无相关性。
我们的数据表明，CD4+CD25highCD127low 诱导痰和 BAL 淋巴细胞可能在活动期肉芽肿性肺疾病和过敏性肺炎患者中发挥作用。活动期 GLD 中 Treg 细胞数量的增加可能参与了活动期肉芽肿性肺疾病的免疫调节。结果表明，分析这些细胞可能作为肉芽肿性肺疾病的疾病活动标志物有用。
