Mroz R M, Korniluk M, Stasiak-Barmuta A, Chyczewska E
Department of Pneumology, Bialystok Medical University, Bialystok, Poland.
J Physiol Pharmacol. 2008 Dec;59 Suppl 6:499-505.
The aim was to find out how the IL-12 and IL-18 levels in the bronchoalveolar lavage fluid (BALF) correspond to the inflammatory activity of the hypersensitivity pneumonitis (HP). We studied 12 patients with HP and 13 normal subjects. IL-12 and IL-18 levels were measured using ELISA kits. We found a significantly higher plasma angiotensin-converting enzyme (ACE) concentration (55 vs. 34 U/L, P=0.0016), lymphocyte percentage (57 vs. 14%, P<0.001), CD8+ cells (32 vs. 17%, P<0.001) and a lower CD4/CD8 ratio (1.2 vs. 2.0, P<0.0001). The IL-12 and IL-18 levels in BALF were significantly higher in HP patients than in healthy subjects (3.9 vs. 3.2 pg/ml, P=0.003 and 14.2 vs. 6.15 pg/ml, P% 0.0001, respectively). We found a strong positive correlation between IL-12 and the percentage of lymphocytes (r=0.68, P=0.015) and a negative one between IL-12 and the percentage of macrophages in BALF (r=-0.64, P=0.024). We conclude that upregulation of the Th1 cell cytokine profile may play a significant role in the pathogenesis of HP.
目的是探究支气管肺泡灌洗液（BALF）中的白细胞介素12（IL-12）和白细胞介素18（IL-18）水平与过敏性肺炎（HP）炎症活动之间的关系。我们研究了12例HP患者和13名正常受试者。使用酶联免疫吸附测定（ELISA）试剂盒测量IL-12和IL-18水平。我们发现血浆血管紧张素转换酶（ACE）浓度显著更高（55比34 U/L，P = 0.0016）、淋巴细胞百分比更高（57比14%，P < 0.001）、CD8 + 细胞更高（32比17%，P < 0.001）以及CD4/CD8比值更低（1.2比2.0，P < 0.0001）。HP患者BALF中的IL-12和IL-18水平显著高于健康受试者（分别为3.9比3.2 pg/ml，P = 0.003和14.2比6.15 pg/ml，P < 0.0001）。我们发现IL-12与淋巴细胞百分比之间存在强正相关（r = 0.68，P = 0.015），而IL-12与BALF中巨噬细胞百分比之间存在负相关（r = -0.64，P = 0.024）。我们得出结论，Th1细胞细胞因子谱的上调可能在HP的发病机制中起重要作用。
