Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Pungnap-2 dong, Songpa-gu, Seoul 138-736, Korea.
J Clin Endocrinol Metab. 2010 Apr;95(4):1869-75. doi: 10.1210/jc.2009-1088. Epub 2010 Feb 15.
Visceral adipose tissue-derived serine protease inhibitor (vaspin) is a novel adipokine with insulin-sensitizing effects. However, the physiological role for vaspin in human metabolic regulation remains to be established.
We studied the 24-h profiles of circulating vaspin concentrations in relation to meal ingestion in normal adults.
Blood samples were drawn 39 times throughout a 24-h period from 10 healthy male subjects provided with meals on a fixed schedule. On a separate day, four subjects were fasted and then provided with an unexpected meal to clarify the effect of meal consumption on serum vaspin levels. Serum vaspin concentrations were determined by ELISA.
Serum vaspin levels were highest in the early morning before breakfast and fell to trough levels within 2 h after breakfast. Serum vaspin levels also showed a preprandial rise and postprandial fall at lunch and dinner, although at lesser degrees than at breakfast. Intermeal vaspin concentrations reached a nadir in the mid-afternoon and showed a nocturnal rise, with peak nighttime vaspin levels being approximately 250% of nadir levels. Unscheduled food ingestion after a prolonged fast significantly reduced serum vaspin levels, suggesting that energy intake itself has a suppressive effect on serum vaspin levels. The diurnal pattern of serum vaspin concentrations was exactly reciprocal to that of insulin and of glucose.
Serum vaspin levels have a meal-related diurnal variation, suggesting a role for vaspin in metabolic regulation. However, the reciprocal relationship between serum vaspin and insulin may negate the importance of vaspin as an physiological insulin sensitizer.
内脏脂肪组织来源的丝氨酸蛋白酶抑制剂（vaspin）是一种具有胰岛素增敏作用的新型脂肪因子。然而，vaspin 在人体代谢调节中的生理作用仍有待确定。
我们研究了正常成年人摄食相关的循环 vaspin 浓度 24 小时变化。
10 名健康男性受试者按照固定的时间表进食，在 24 小时内共抽取 39 次血样。在另一天，4 名受试者禁食，然后给予意外的餐食，以明确摄食对血清 vaspin 水平的影响。通过 ELISA 测定血清 vaspin 浓度。
血清 vaspin 水平在早餐前的清晨最高，早餐后 2 小时内降至低谷。血清 vaspin 水平在午餐和晚餐时也出现餐前升高和餐后下降，但程度低于早餐时。两餐之间的 vaspin 浓度在下午中段达到最低点，并出现夜间升高，夜间最高 vaspin 水平约为最低点的 250%。长时间禁食后不规律的摄食明显降低了血清 vaspin 水平，提示能量摄入本身对血清 vaspin 水平有抑制作用。血清 vaspin 浓度的昼夜节律与胰岛素和葡萄糖的昼夜节律完全相反。
血清 vaspin 水平存在与摄食相关的昼夜变化，提示 vaspin 在代谢调节中起作用。然而，血清 vaspin 与胰岛素之间的反向关系可能否定了 vaspin 作为生理胰岛素增敏剂的重要性。
