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驯服下一代猛兽。

Taming the next-gen beast.

机构信息

Stephen Bentley is at the Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.

出版信息

Nat Rev Microbiol. 2010 Mar;8(3):161. doi: 10.1038/nrmicro2322.

DOI:10.1038/nrmicro2322
PMID:20157335
Abstract

This month's Genome Watch discusses how alternative approaches to using second-generation sequencing technologies are powerful tools for the analysis of common pathogenic bacteria.

摘要

本期的《基因组观察》讨论了如何采用第二代测序技术的替代方法,这些方法是分析常见病原体的有力工具。

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Genomic variability of monkeypox virus among humans, Democratic Republic of the Congo.

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Evolution of MRSA during hospital transmission and intercontinental spread.耐甲氧西林金黄色葡萄球菌(MRSA)在医院传播和洲际传播过程中的进化。
Science. 2010 Jan 22;327(5964):469-74. doi: 10.1126/science.1182395.
2
Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants.利用一百万转座子突变体同时检测每一个伤寒沙门氏菌基因。
Genome Res. 2009 Dec;19(12):2308-16. doi: 10.1101/gr.097097.109. Epub 2009 Oct 13.
3
Tracking insertion mutants within libraries by deep sequencing and a genome-wide screen for Haemophilus genes required in the lung.
刚果民主共和国人类中猴痘病毒的基因组变异性
Emerg Infect Dis. 2014 Feb;20(2):232-9. doi: 10.3201/eid2002.130118.
4
Analysis of quality raw data of second generation sequencers with Quality Assessment Software.使用质量评估软件对第二代测序仪的高质量原始数据进行分析。
BMC Res Notes. 2011 Apr 18;4:130. doi: 10.1186/1756-0500-4-130.
通过深度测序追踪文库中的插入突变体,并对肺部所需的嗜血杆菌基因进行全基因组筛选。
Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16422-7. doi: 10.1073/pnas.0906627106. Epub 2009 Sep 4.
4
Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms.转座子测序(Tn-seq):用于微生物适应性和遗传相互作用研究的高通量平行测序技术
Nat Methods. 2009 Oct;6(10):767-72. doi: 10.1038/nmeth.1377. Epub 2009 Sep 20.
5
Identifying genetic determinants needed to establish a human gut symbiont in its habitat.确定在其栖息地建立人类肠道共生体所需的遗传决定因素。
Cell Host Microbe. 2009 Sep 17;6(3):279-89. doi: 10.1016/j.chom.2009.08.003.