Department of Anatomy and Research Center for Tumor Immunology, Inje University College of Medicine, Busan 614-735, Korea.
Immune Netw. 2009 Dec;9(6):236-42. doi: 10.4110/in.2009.9.6.236. Epub 2009 Dec 31.
Melanoma is the most fatal form of skin cancer due to its rapid metastasis. Recently, several studies reported that selenium can induce apoptosis in melanoma cells. However, the precise mechanism remains to be elucidated. In this study, we investigated the effect of selenium on cell proliferation in murine melanoma and on tumor growth and metastasis in C57BL/6 mice.
Cell proliferation was measured by MTT assay in selenium-treated melanoma cells. Cell cycle distribution was analysized by staining DNA with propidum iodide (PI). mRNA and protein expression related to cell cycle arrest was measured by reverse transcription PCR and western blot. Tumor growth and metastasis was measured by in vivo model.
Selenium was suppressed the proliferation of melanoma cells in a dose dependent manner. The growth inhibition of melanoma by selenium was associated with an arrest of cell cycle distribution at G0/G1 stage. The mRNA and protein level of CDK2/CDK4 was suppressed by treatment with selenium in a time-dependent manner. In vivo, tumor growth was not suppressed by selenium; however tumor metastasis was suppressed by selenium in mouse model.
These results suggest that selenium might be a potent agent to inhibit proliferative activity of melanoma cells.
黑色素瘤是皮肤癌中最致命的形式，因为它转移迅速。最近，有几项研究报告称，硒可以诱导黑色素瘤细胞凋亡。然而，其确切的机制仍有待阐明。在这项研究中，我们研究了硒对小鼠黑色素瘤细胞增殖以及 C57BL/6 小鼠肿瘤生长和转移的影响。
用 MTT 法测定硒处理的黑色素瘤细胞的细胞增殖。用碘化丙啶（PI）染色 DNA 分析细胞周期分布。用逆转录 PCR 和 Western blot 测定与细胞周期阻滞相关的 mRNA 和蛋白表达。通过体内模型测量肿瘤生长和转移。
硒呈剂量依赖性抑制黑色素瘤细胞的增殖。硒对黑色素瘤的生长抑制与细胞周期阻滞在 G0/G1 期有关。用硒处理后，CDK2/CDK4 的 mRNA 和蛋白水平呈时间依赖性下降。在体内，硒不能抑制肿瘤生长；然而，硒能抑制小鼠模型中的肿瘤转移。
这些结果表明，硒可能是一种抑制黑色素瘤细胞增殖活性的有效药物。
