Galectin-1 的过表达与口腔鳞状细胞癌的病理分化程度呈负相关。
Overexpression of Galectin-1 is negatively correlated with pathologic differentiation grade in oral squamous cell carcinoma.
机构信息
Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, School of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, People's Republic of China.
出版信息
J Cancer Res Clin Oncol. 2010 Oct;136(10):1527-35. doi: 10.1007/s00432-010-0810-2. Epub 2010 Feb 16.
PURPOSE
To determine the Galectin-1 protein expression in oral squamous cell carcinoma (OSCC).
METHODS
Comparative proteomic analysis of an in vitro cellular carcinogenesis model of OSCC we previously established was performed to identify differentially expressed proteins. Galectin-1 was further validated in vitro (human immortalized oral epithelia cell line and OSCC lines) and in vivo (tissue samples from OSCC patients) by Western blotting and immunohistochemistry, respectively.
RESULTS
Increased Galectin-1 protein expression was identified in the cancerous cell line compared with the immortalized oral epithelial cell line in the in vitro cellular carcinogenesis model, and then validated in the OSCC lines and cancerous tissues. Galectin-1 protein expression was negatively correlated with the tumor pathologic differentiation grades, a higher Galectin-1 protein expression indicating a poorer pathologic differentiation grade.
CONCLUSIONS
Galectin-1 protein expression level increases in OSCC, it may serve as a candidate marker for pathologic differentiation grade of OSCC.
目的
检测口腔鳞状细胞癌（OSCC）中半乳糖凝集素-1 蛋白的表达。
方法
采用比较蛋白质组学分析方法，对我们之前建立的口腔鳞状细胞癌体外细胞癌变模型中的差异表达蛋白进行鉴定。通过 Western blot 和免疫组织化学法，分别在体外（人永生化口腔上皮细胞系和 OSCC 细胞系）和体内（OSCC 患者的组织样本）进一步验证半乳糖凝集素-1。
结果
在体外细胞癌变模型中，与永生化口腔上皮细胞系相比，癌变细胞系中半乳糖凝集素-1 蛋白表达增加，并在 OSCC 细胞系和癌变组织中得到验证。半乳糖凝集素-1 蛋白表达与肿瘤病理分化程度呈负相关，即半乳糖凝集素-1 蛋白表达越高，病理分化程度越差。
结论
半乳糖凝集素-1 蛋白在 OSCC 中表达增加，可能成为 OSCC 病理分化程度的候选标志物。
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