生物界面——PLL/HA 厚膜与纳米粒子和微胶囊的相互作用。

Bio-interfaces--interaction of PLL/HA thick films with nanoparticles and microcapsules.

机构信息

Department of Interfaces, Max-Planck Institute of Colloids and Interfaces, Am Muehlenberg 1, Research Campus Golm, Potsdam D-14424, Germany.

出版信息

Chemphyschem. 2010 Mar 15;11(4):822-9. doi: 10.1002/cphc.200900676.

DOI:10.1002/cphc.200900676

PMID:20157913

Abstract

The interaction of biocompatible, exponentially grown films composed of poly-L-lysine (PLL) and hyaluronic acid (HA) polymers with gold nanoparticles and microcapsules is studied. Both aggregated and non-aggregated nanoparticle states are achieved; desorption of PLL accounts for aggregation of nanoparticles. The presence of aggregates of gold nanoparticles on films enables remote activation by near-infrared irradiation due to local, nanometer confined heating. Thermally shrunk microcapsules, which are remarkably monodisperse upon preparation but gain polydispersity after months of storage, are also adsorbed onto films. PLL polymers desorbed from films interact with microcapsules introducing a charge imbalance which leads to an increase of the microcapsule size, thus films amplify this effect. Multifunctional, biocompatible, thick gel films with remote activation and release capabilities are targeted for cell cultures in biology and tissue engineering in medicine.

摘要

研究了由聚-L-赖氨酸（PLL）和透明质酸（HA）聚合物组成的具有生物相容性、指数生长的薄膜与金纳米粒子和微胶囊的相互作用。实现了聚集和非聚集的纳米粒子状态；PLL 的解吸导致纳米粒子的聚集。金纳米粒子在薄膜上的聚集存在使得能够通过近红外辐射进行远程激活，因为存在局部纳米受限加热。在制备时具有显著单分散性但在数月的储存后获得多分散性的热收缩微胶囊也被吸附到薄膜上。从薄膜中解吸的 PLL 聚合物与微胶囊相互作用，引入电荷不平衡，导致微胶囊尺寸增加，从而使薄膜放大了这种效应。目标是为生物学中的细胞培养和医学中的组织工程制备具有远程激活和释放功能的多功能、生物相容的厚凝胶薄膜。