Universidade de Franca, Av. Dr. Armando Salles de Oliveira, 201, CEP 14404-600, Franca, São Paulo, Brazil.
Z Naturforsch C J Biosci. 2009 Nov-Dec;64(11-12):793-7. doi: 10.1515/znc-2009-11-1207.
Chemical investigation of the EtOAc fraction (EF) obtained from the ethanolic extract of Zanthoxylum naranjillo (Rutaceae) leaves (EE) by preparative HPLC resulted in the isolation of protocatechuic acid (1), gallic acid (2), p-hydroxybenzoic acid (3), and 5-O-caffeoylshikimic acid (4). This is the first time that the presence of compounds 1-4 in Z. naranjillo has been reported. Compounds 1-4, the EE, and EF were tested in vitro against Schistosoma mansoni adult worms. The results showed that the S. mansoni daily egg production decreased by 29.8%, 13.5%, 28.4%, 17.7%, 16.3%, and 6.4%, respectively. Compounds 1 and 3 were also able to separate adult worm pairs into male and female. This activity may be correlated with the reduction in egg production, since 1 and 3 showed better inhibitory properties compared with 2 and 4.
从芸香科植物纳拉尼洛（Zanthoxylum naranjillo）叶的乙醇提取物（EE）中用制备高效液相色谱法（HPLC）得到的乙酸乙酯（EtOAc）部分（EF）的化学研究导致分离出原儿茶酸（1）、没食子酸（2）、对羟基苯甲酸（3）和 5-O-咖啡酰莽草酸（4）。这是首次报道纳拉尼洛中存在化合物 1-4。化合物 1-4、EE 和 EF 在体外对曼氏血吸虫成虫进行了测试。结果表明，S. mansoni 的每日产卵量分别减少了 29.8%、13.5%、28.4%、17.7%、16.3%和 6.4%。化合物 1 和 3 还能够将成虫对分离成雄性和雌性。这种活性可能与产卵减少有关，因为与化合物 2 和 4 相比，1 和 3 显示出更好的抑制特性。
