家鸡艾美耳球虫：通过场反转凝胶电泳解析染色体
Eimeria spp. of the domestic fowl: resolution of chromosomes by field inversion gel electrophoresis.
作者信息
Fernando M A, Pasternak J J
机构信息
Department of Pathology, University of Guelph, Ontario, Canada.
出版信息
Exp Parasitol. 1991 Apr;72(3):306-10. doi: 10.1016/0014-4894(91)90150-u.
The molecular karyotypes of five species of chicken coccidia, viz., Eimeria acervulina, E. brunetti, E. maxima, E. necatrix, and E. tenella, were determined using field inversion gel electrophoresis (FIGE). Each species has a distinctive set of resolvable chromosomes which range from about 1 to greater than 5.7 megabases. We were able to resolve at least 8 chromosomes for E. acervulina, 5 for E. brunetti, 10 for E. maxima, 6 for E. necatrix, and 9 for E. tenella. If the value of 67 megabases for the genomic DNA of E. tenella is accurate, then under the conditions used here only about 60% of its chromosomal complement has been resolved.
利用脉冲场反转凝胶电泳（FIGE）测定了五种鸡球虫的分子核型，即堆型艾美耳球虫、布氏艾美耳球虫、巨型艾美耳球虫、毒害艾美耳球虫和柔嫩艾美耳球虫。每个物种都有一组独特的可分辨染色体，大小范围从约1兆碱基到大于5.7兆碱基。我们能够分辨出堆型艾美耳球虫至少8条染色体、布氏艾美耳球虫5条、巨型艾美耳球虫10条、毒害艾美耳球虫6条和柔嫩艾美耳球虫9条。如果柔嫩艾美耳球虫基因组DNA的67兆碱基这一数值准确，那么在此处使用的条件下，其染色体组中只有约60%得到了分辨。
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