Young A A, Mott D M, Stone K, Cooper G J
Amylin Corporation, San Diego, CA 92121.
FEBS Lett. 1991 Apr 9;281(1-2):149-51. doi: 10.1016/0014-5793(91)80380-l.
The pancreatic beta-cell hormone amylin acts in isolated rat skeletal muscle to decrease insulin-stimulated incorporation of glucose into glycogen. It also increases blood levels of lactate and glucose in fasted rats in vivo. However, it remained uncertain whether amylin exerts direct effects to stimulate muscle glycogenolysis. We now report that amylin caused a dose-dependent increase in activity of muscle glycogen phosphorylase in isolated rat soleus muscle by stimulating phosphorylase a. Insulin inhibited amylin-stimulated activation of phosphorylase. Effects of amylin to stimulate muscle glycogenolysis are consistent with observed effects of amylin in vivo and could be a major mechanism whereby amylin modulates carbohydrate metabolism.
胰腺β细胞激素胰淀素在离体大鼠骨骼肌中发挥作用，可降低胰岛素刺激的葡萄糖转化为糖原的过程。在体内，它还会使禁食大鼠的乳酸和葡萄糖血水平升高。然而，胰淀素是否直接发挥作用刺激肌肉糖原分解仍不明确。我们现在报告，胰淀素通过刺激磷酸化酶a，使离体大鼠比目鱼肌中肌肉糖原磷酸化酶的活性呈剂量依赖性增加。胰岛素抑制胰淀素刺激的磷酸化酶激活。胰淀素刺激肌肉糖原分解的作用与在体内观察到的胰淀素作用一致，可能是胰淀素调节碳水化合物代谢的主要机制。
