通过CD63或CD203c检测嗜碱性粒细胞敏感性是特异性免疫疗法的一项功能指标。
Basophil sensitivity through CD63 or CD203c is a functional measure for specific immunotherapy.
作者信息
Mikkelsen Susan, Bibby Bo Martin, Dolberg Mette Konow Bøgebjerg, Dahl Ronald, Hoffmann Hans Jürgen
机构信息
Department of Respiratory Diseases, Aarhus University Hospital, Aarhus, Denmark.
出版信息
Clin Mol Allergy. 2010 Feb 16;8(1):2. doi: 10.1186/1476-7961-8-2.
BACKGROUND
Subcutaneous Immunotherapy (SCIT) modifies the allergic response and relieves allergic symptoms. SCIT is the only and a very effective treatment for insect venom allergy. We hypothesized that basophil sensitivity, measured through the basophil activation test, would decrease during SCIT up dosing. Expression of CD203c was compared to CD63 as marker for basophil activation, using a Bland Altman plot and ROC curves.
METHODS
Patients (n = 18) starting subcutaneous SCIT for wasp allergy with an up dosing scheme of 7 to 11 weeks were enrolled. Heparinised blood samples were drawn at weeks 1-4, 7 and at the first maintenance visit. Basophils were stimulated at 7 log dilutions of V. vespula allergen for 15 min, and were stained with CD203c and CD63. Basophils were identified as CD203c+ leukocytes, and the proportion of CD63+ and CD203c+ cells were plotted against allergen concentration. A sigmoid curve was fitted to the points, and the allergen concentration at which half of the maximal activation was achieved, LC50, was calculated. In another series of experiments, LC50 calculated in whole blood (AP) was subtracted from LC50 calculated with basophils suspended in plasma from a nonatopic donor (HS) to determine the protective effect of soluble factors in blood of patients treated with SCIT.
RESULTS
Heparin blood basophil activation was similar through CD63 and CD203c. Basophils were significantly more sensitized three weeks after initiation of SCIT compared to baseline (p < 0,01). The difference in LC50 increased by 1,04 LC50 units (p = 0,04) in patients that had just achieved maintenance dose compared with patients before initiating SCIT. When maintenance allergen concentrations had been reached, an increase in the protective plasma component was documented. Blood basophil concentration was marginally reduced by SCIT.
CONCLUSION
Basophil activation is a versatile and sensitive tool that measures changes in the humoral immune response to allergen during SCIT.
背景
皮下免疫疗法（SCIT）可改变过敏反应并缓解过敏症状。SCIT是治疗昆虫毒液过敏的唯一且非常有效的方法。我们假设，通过嗜碱性粒细胞活化试验测得的嗜碱性粒细胞敏感性在SCIT剂量递增期间会降低。使用布兰德-奥特曼图和ROC曲线，将CD203c的表达与作为嗜碱性粒细胞活化标志物的CD63进行比较。
方法
招募了18例开始采用7至11周剂量递增方案进行黄蜂过敏皮下SCIT治疗的患者。在第1 - 4周、第7周和首次维持访视时采集肝素化血样。用7种对数稀释度的黄蜂过敏原刺激嗜碱性粒细胞15分钟，并用CD203c和CD63进行染色。将嗜碱性粒细胞鉴定为CD203c +白细胞，并将CD63 +和CD203c +细胞的比例与过敏原浓度作图。对这些点拟合一条S形曲线，并计算达到最大活化一半时的过敏原浓度，即LC50。在另一系列实验中，从用非特应性供体血浆悬浮的嗜碱性粒细胞计算出的LC50中减去全血（AP）计算出的LC50，以确定SCIT治疗患者血液中可溶性因子的保护作用。
结果
通过CD63和CD203c检测肝素化血液嗜碱性粒细胞活化情况相似。与基线相比，SCIT开始后三周嗜碱性粒细胞的致敏性显著更高（p < 0.01）。与开始SCIT前的患者相比，刚达到维持剂量的患者的LC50差异增加了1.04个LC50单位（p = 0.04）。当达到维持过敏原浓度时，记录到保护性血浆成分增加。SCIT使血液嗜碱性粒细胞浓度略有降低。
结论
嗜碱性粒细胞活化是一种通用且敏感的工具，可测量SCIT期间对过敏原的体液免疫反应变化。
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