冰晶形成对于组织和器官的冷冻保存的相关性。

The relevance of ice crystal formation for the cryopreservation of tissues and organs.

机构信息

Department of Biology, University of York, YO10 5YW, UK.

出版信息

Cryobiology. 2010 Jul;60(3 Suppl):S36-44. doi: 10.1016/j.cryobiol.2010.02.003. Epub 2010 Feb 14.

DOI:10.1016/j.cryobiol.2010.02.003

PMID:20159009

Abstract

This paper discusses the role of ice crystal formation in causing or contributing to the difficulties that have been encountered in attempts to develop effective methods for the cryopreservation of some tissues and all organs. It is shown that extracellular ice can be severely damaging but also that cells in situ in tissues can behave quite differently from similar cells in a suspension with respect to intracellular freezing. It is concluded that techniques that avoid the formation of ice altogether are most likely to yield effective methods for the cryopreservation of recalcitrant tissues and vascularised organs.

摘要

本文讨论了冰晶形成在导致或促成尝试开发有效方法冷冻保存某些组织和所有器官时所遇到的困难方面的作用。研究表明，细胞外的冰可能会造成严重的损害，但组织中处于原位的细胞与悬浮液中的类似细胞相比，其细胞内冷冻的行为可能会有很大的不同。本文的结论是，完全避免冰晶形成的技术最有可能为冷冻保存顽固组织和有血管化的器官提供有效的方法。