Departmento de Física and Center for Advanced Interdisciplinary Research in Materials, Universidad de Santiago de Chile, Santiago, Chile.
Biophys J. 2010 Feb 17;98(4):515-23. doi: 10.1016/j.bpj.2009.10.024.
We investigate the mechanical response of PC12 neurites subjected to a drag force imposed by a laminar flow perpendicular to the neurite axis. The curvature of the catenary shape acquired by an initially straight neurite under the action of the drag force provides information on both elongation and tension of the neurite. This method allows us to measure the rest tension and viscoelastic parameters of PC12 neurites and active behavior of neurites. Measurement of oscillations in the strain rate of neurites at constant flow rate provides insight on the response of molecular motors and additional support for the presence of a negative strain-rate sensitivity region in the global mechanical response of PC12 neurites.
我们研究了 PC12 神经突在垂直于神经突轴的层流拖拽力作用下的力学响应。在拖拽力作用下，初始为直线的神经突呈现出的悬链线形状的曲率，提供了关于神经突伸长和张力的信息。该方法可用于测量 PC12 神经突的静张力和黏弹参数，以及神经突的活性行为。在恒定流速下测量神经突应变速率的振荡，可深入了解分子马达的响应，并为 PC12 神经突整体力学响应中存在负应变速率敏感区提供更多支持。
