Ward C M, Morgan M R
AFRC Institude of Food Research, Norwich, UK.
Food Addit Contam. 1991 Jan-Feb;8(1):9-15. doi: 10.1080/02652039109373951.
The reproducibility of a commercially available kit utilising enzyme-linked immunosorbent assay (ELISA) for determination of total aflatoxins has been analysed. Peanut butter samples contaminated with aflatoxin at three concentrations (mean 5.8, 11.7 and 24.8 ng/g) were analysed on different occasions using four kits. Coefficients of variation at all concentrations ranged from 2.6% to 13.6% within replicate, 0.3% to 16.2% within plate and 6.7% to 10.2% between kits. Standard curve precision profiles showed a minimum coefficient variation (CV) of 2-4% and a working range (below 10% CV) covering almost the entire standard curve.
已对一种利用酶联免疫吸附测定法（ELISA）测定总黄曲霉毒素的市售试剂盒的重现性进行了分析。使用四个试剂盒在不同时间对黄曲霉毒素污染程度为三种浓度（平均5.8、11.7和24.8 ng/g）的花生酱样品进行了分析。所有浓度下的变异系数在重复测定中为2.6%至13.6%，在平板内为0.3%至16.2%，在试剂盒之间为6.7%至10.2%。标准曲线精密度图谱显示最小变异系数（CV）为2 - 4%，工作范围（CV低于10%）几乎覆盖整个标准曲线。
