Krukowski Sylwester, Sztelmach Konrad
Medical University of Warsaw, Faculty of Pharmacy, Department of Analytical Chemistry Warsaw Poland
RSC Adv. 2022 Aug 22;12(37):23769-23777. doi: 10.1039/d2ra03372k.
The modifications carried out as part of this study were aimed at examining the effect of the addition of collagen amino acids: glycine, proline and hydroxyproline (used separately and in binary mixtures) on the physicochemical properties of hydroxyapatite obtained in their presence . The influence of mixtures of amino acids on these properties is an important element of scientific novelty. The obtained samples were tested with the use of instrumental methods: FT-IR, TEM, EDXMA, PXRD and UV spectrophotometry. The results showed the influence of the amino acids used on changes in the relative content of the labile phosphate groups constituting the structure of the hydrated surface layer of crystals. As a consequence, there were differences in some physicochemical properties of the obtained hydroxyapatites (degree of crystallinity, molar Ca/P ratio). It was also determined how the ability of the used amino acids to bind to hydroxyapatite changes.
作为本研究的一部分所进行的改性旨在研究添加胶原蛋白氨基酸（甘氨酸、脯氨酸和羟脯氨酸，单独使用及二元混合物形式）对在其存在下获得的羟基磷灰石物理化学性质的影响。氨基酸混合物对这些性质的影响是科学新颖性的一个重要因素。使用仪器方法（傅里叶变换红外光谱法、透射电子显微镜、能量散射X射线能谱分析、粉末X射线衍射和紫外分光光度法）对所得样品进行了测试。结果表明，所使用的氨基酸对构成晶体水合表面层结构的不稳定磷酸基团相对含量变化有影响。因此，所得羟基磷灰石的一些物理化学性质（结晶度、摩尔钙/磷比）存在差异。还确定了所使用的氨基酸与羟基磷灰石结合能力的变化情况。
