Department of Oral Biology, Indiana University School of Dentistry, 1121 West Michigan Street, DS 271, Indianapolis, IN 46202, USA.
J Dent Res. 2010 May;89(5):527-31. doi: 10.1177/0022034509358567. Epub 2010 Feb 16.
Cigarette smoke condensate (CSC) is the particulate matter of cigarette smoke. Porphyromonas gingivalis (P. gingivalis) is an opportunistic pathogen involved in periodontitis. It was hypothesized that the combination of CSC and P. gingivalis would increase the collagen-degrading ability of human gingival fibroblasts (HGFs). In this study, HGFs were exposed to CSC, P. gingivalis supernatant, and CSC plus P. gingivalis supernatant. The collagen-degrading ability and protein/mRNA levels of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) of HGFs were examined. The combined treatment increased collagen degradation, protein levels of active forms of MMP-1, MMP-2, MMP-3, and MMP-14 in conditioned media, and the low-molecular-weight fragment of MMP-14 in membrane extracts, as well as mRNA levels of MMP-1, MMP-2, and MMP-14. In conclusion, the combined effects of CSC and P. gingivalis increased HGF-mediated collagen degradation by destroying the balance between MMPs and TIMPs at multiple levels.
香烟烟雾冷凝物（CSC）是香烟烟雾的颗粒物质。牙龈卟啉单胞菌（P. gingivalis）是一种与牙周炎有关的机会性病原体。本研究假设 CSC 和 P. gingivalis 的联合作用会增加人牙龈成纤维细胞（HGFs）的胶原降解能力。在这项研究中，HGFs 暴露于 CSC、P. gingivalis 上清液和 CSC 加 P. gingivalis 上清液中。检测了 HGFs 的胶原降解能力以及基质金属蛋白酶（MMPs）和金属蛋白酶组织抑制剂（TIMPs）的蛋白/ mRNA 水平。联合处理增加了条件培养基中 MMP-1、MMP-2、MMP-3 和 MMP-14 的活性形式以及膜提取物中 MMP-14 的低分子量片段的胶原降解，以及 MMP-1、MMP-2 和 MMP-14 的 mRNA 水平。总之，CSC 和 P. gingivalis 的联合作用通过在多个水平破坏 MMPs 和 TIMPs 之间的平衡，增加了 HGF 介导的胶原降解。
