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吉姆萨染色带型后的染色体原位抑制杂交

Chromosomal in situ suppression hybridization after Giemsa banding.

作者信息

Klever M, Grond-Ginsbach C, Scherthan H, Schroeder-Kurth T M

机构信息

Institut für Humangenetik und Anthropologie der Universität, Heidelberg, Federal Republic of Germany.

出版信息

Hum Genet. 1991 Mar;86(5):484-6. doi: 10.1007/BF00194638.

DOI:10.1007/BF00194638
PMID:2016088
Abstract

We report the successive application of classical Giemsa banding and chromosomal in situ suppression hybridization in clinical cytogenetics. The use of both techniques within one protocol requires an additional fixation of the chromosome preparations and an improved suppression of the labelled repetitive sequences. The combination of these two cytological techniques allows the high resolution mapping of translocated Y-chromosomal sequences in the chromosome set of an XX-male.

摘要

我们报告了经典吉姆萨显带和染色体原位抑制杂交技术在临床细胞遗传学中的连续应用。在同一实验方案中使用这两种技术需要对染色体标本进行额外固定，并改进对标记重复序列的抑制。这两种细胞学技术的结合使得能够在XX男性的染色体组中对易位的Y染色体序列进行高分辨率定位。

相似文献

1
Chromosomal in situ suppression hybridization after Giemsa banding.吉姆萨染色带型后的染色体原位抑制杂交
Hum Genet. 1991 Mar;86(5):484-6. doi: 10.1007/BF00194638.
2
[Detection of complex chromosomal aberrations in patients with multiple myeloma using multiplex fluorescence in situ hybridization].[运用多重荧光原位杂交技术检测多发性骨髓瘤患者的复杂染色体畸变]
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Aug;27(4):441-4. doi: 10.3760/cma.j.issn.1003-9406.2010.04.018.
3
Further cytologic evidence for Xp-Yp translocation in XX males using in situ hybridization with Y-derived probe.使用Y衍生探针原位杂交对XX男性中Xp-Yp易位的进一步细胞学证据。
Hum Genet. 1987 Mar;75(3):228-33. doi: 10.1007/BF00281064.
4
Chromosomal in situ suppression hybridization of human gonosomes and autosomes and its use in clinical cytogenetics.人类性染色体和常染色体的染色体原位抑制杂交及其在临床细胞遗传学中的应用。
Hum Genet. 1990 Jul;85(2):145-50. doi: 10.1007/BF00193186.
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Traditional and molecular cytogenetics.传统与分子细胞遗传学
J Reprod Med. 1992 Jun;37(6):485-98.
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Molecular detection of a translocation (Y;15) in a 45,X male.一名45,X男性中(Y;15)易位的分子检测。
Hum Genet. 1986 Dec;74(4):372-7. doi: 10.1007/BF00280488.
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Nonrandom inactivation of the Y-bearing X chromosome in a 46,XX individual: evidence for the etiology of 46,XX true hermaphroditism.46,XX个体中携带Y染色体的X染色体的非随机失活：46,XX真性两性畸形病因的证据。
Cytogenet Cell Genet. 1994;66(1):22-6. doi: 10.1159/000133656.
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A 45,X male with molecular evidence of a translocation of Y euchromatin onto chromosome 1.一名45,X男性，有Y常染色质易位至1号染色体的分子证据。
Hum Genet. 1990 Nov;86(1):94-8. doi: 10.1007/BF00205184.
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Chromosomal rearrangements detected by FISH and G-banding.通过荧光原位杂交（FISH）和G显带检测到的染色体重排。
J Formos Med Assoc. 1996 Sep;95(9):686-91.
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Molecular detection of a translocation (Y;11) (q11.2;q24) in a 45,X male with signs of Jacobsen syndrome.在一名具有雅各布森综合征体征的45,X男性中检测到(Y;11)(q11.2;q24)易位的分子特征。
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Mapping chromosomal homology between humans and the black-handed spider monkey by fluorescence in situ hybridization.

本文引用的文献

1
Counterstain-enhanced chromosome banding.复染增强染色体显带
Hum Genet. 1981;57(1):1-14.
2
Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and beta-NGF genes.新型非同位素原位杂交技术可检测常规G显带人类染色体中的小（1千碱基）独特序列：N-myc和β-神经生长因子基因的精细定位
Nucleic Acids Res. 1987 Jun 25;15(12):4761-70. doi: 10.1093/nar/15.12.4761.
3
Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-specific library probes.
通过荧光原位杂交技术绘制人类与黑掌蜘蛛猴之间的染色体同源性图谱。
Chromosome Res. 1997 Dec;5(8):527-36. doi: 10.1023/a:1018489602312.
4
Sequential G-banding FISH on human sperm chromosomes.
Chromosome Res. 1997 Nov;5(7):457-61. doi: 10.1023/a:1018464929628.
5
Variegated transgene expression in mouse mammary gland is determined by the transgene integration locus.小鼠乳腺中斑驳的转基因表达由转基因整合位点决定。
Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6659-64. doi: 10.1073/pnas.93.13.6659.
6
A complex chromosomal rearrangement detected prenatally and studied by fluorescence in situ hybridization.产前检测到的一种复杂染色体重排，并通过荧光原位杂交进行研究。
Hum Genet. 1993 Sep;92(2):117-21. doi: 10.1007/BF00219677.
7
Localisation of the human nuclear factor I/X (NFI/X) gene to chromosome 19p13 and detection of five other related loci at 1p21-22, 1q42-43, 5q15, 11p13 and 20q13 by FISH.
Hum Genet. 1993 Jul;91(6):535-7. doi: 10.1007/BF00205076.
8
Modes of DAPI banding and simultaneous in situ hybridization.DAPI显带及同步原位杂交模式。
Chromosoma. 1993 May;102(5):325-32. doi: 10.1007/BF00661275.
9
Maternal cell contamination in amniotic fluid samples as a consequence of the sampling technique.由于采样技术导致羊水样本中出现母源细胞污染。
Hum Genet. 1994 Feb;93(2):121-4. doi: 10.1007/BF00210594.
10
The mapping of transgenes by fluorescence in situ hybridization on G-banded mouse chromosomes.
Mamm Genome. 1994 Jun;5(6):337-41. doi: 10.1007/BF00356551.
使用染色体特异性文库探针通过原位杂交检测中期和间期肿瘤细胞中的染色体畸变。
Hum Genet. 1988 Nov;80(3):235-46. doi: 10.1007/BF01790091.
4
Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries.利用重组DNA文库通过原位抑制杂交技术描绘中期和间期细胞中的单个人类染色体。
Hum Genet. 1988 Nov;80(3):224-34. doi: 10.1007/BF01790090.
5
Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.使用人类染色体特异性文库进行荧光原位杂交：检测21三体和4号染色体易位
Proc Natl Acad Sci U S A. 1988 Dec;85(23):9138-42. doi: 10.1073/pnas.85.23.9138.
6
Interspecies relationship of a repetitive chromosome-specific DNA.一种重复性染色体特异性DNA的种间关系。
Hereditas. 1989;111(2):183-8. doi: 10.1111/j.1601-5223.1989.tb00394.x.
7
Detection of chromosome aberrations in interphase tumor nuclei by nonradioactive in situ hybridization.通过非放射性原位杂交检测间期肿瘤细胞核中的染色体畸变。
Cancer Genet Cytogenet. 1989 Oct 1;42(1):87-98. doi: 10.1016/0165-4608(89)90011-3.
8
Chromosomal in situ suppression hybridization of human gonosomes and autosomes and its use in clinical cytogenetics.人类性染色体和常染色体的染色体原位抑制杂交及其在临床细胞遗传学中的应用。
Hum Genet. 1990 Jul;85(2):145-50. doi: 10.1007/BF00193186.