Karunatilaka Krishanthi S, Rueda David
Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202, USA.
Chem Phys Lett. 2009 Jul 1;476(1):1-10. doi: 10.1016/j.cplett.2009.06.001.
Over the past decade, single-molecule fluorescence studies have elucidated the structure-function relationship of RNA molecules. The real-time observation of individual RNAs by single-molecule fluorescence has unveiled the dynamic behavior of complex RNA systems in unprecedented detail, revealing the presence of transient intermediate states and their kinetic pathways. This review provides an overview of how single-molecule fluorescence has been used to explore the dynamics of RNA folding and catalysis.
在过去十年中，单分子荧光研究阐明了RNA分子的结构-功能关系。通过单分子荧光对单个RNA进行实时观察，以前所未有的细节揭示了复杂RNA系统的动态行为，揭示了瞬时中间态的存在及其动力学途径。本文综述了单分子荧光如何用于探索RNA折叠和催化的动力学。
