Chikkaveeraiah Bhaskara V, Liu Hongyun, Mani Vigneshwaran, Papadimitrakopoulos Fotios, Rusling James F
Department of Chemistry and Institute of Materials Science, University of Connecticut, Storrs, CT 06269.
Electrochem commun. 2009 Apr 1;11(4):819-822. doi: 10.1016/j.elecom.2009.02.002.
We report herein a simple device for rapid biosensing consisting of a single microfluidic channel made from poly(dimethylsiloxane) (PDMS) coupled to an injector, and incorporating a biocatalytic sensing electrode, reference and counter electrodes. The sensing electrode was a gold wire coated with 5 nm glutathione-decorated gold nanoparticles (AuNPs). Sensitive detection of H(2)O(2) based on direct bioelectrocatalysis by horseradish peroxidase (HRP) was used for evaluation. HRP was covalently linked the glutathione-AuNPs. This electrode presented quasi-reversible cyclic voltammetry peaks at -0.01 V vs Ag/AgCl at pH 6.5 for the HRP heme Fe(III)/Fe(II) couple. Direct electrochemical activity of HRP was used to detect H(2)O(2) at high sensitivity with a detection limit of 5 nM in an unmediated system.
我们在此报告一种用于快速生物传感的简单装置，它由一个由聚二甲基硅氧烷（PDMS）制成的单个微流体通道与一个注射器相连组成，并包含一个生物催化传感电极、参比电极和对电极。传感电极是一根涂有5纳米谷胱甘肽修饰金纳米颗粒（AuNPs）的金线。基于辣根过氧化物酶（HRP）的直接生物电催化对H₂O₂进行灵敏检测用于评估。HRP与谷胱甘肽 - AuNPs共价连接。在pH 6.5时，该电极对于HRP血红素Fe(III)/Fe(II)电对在相对于Ag/AgCl为 - 0.01 V处呈现准可逆循环伏安峰。HRP的直接电化学活性用于在无媒介体系中以5 nM的检测限高灵敏度检测H₂O₂。
