Bishop Gregory W, Satterwhite Jennifer E, Bhakta Snehasis, Kadimisetty Karteek, Gillette Kelsey M, Chen Eric, Rusling James F
†Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060, United States.
‡Institute of Materials Science, University of Connecticut, Storrs, Connecticut 06269-3136, United States.
Anal Chem. 2015;87(10):5437-43. doi: 10.1021/acs.analchem.5b00903. Epub 2015 May 1.
A consumer-grade fused filament fabrication (FFF) 3D printer was used to construct fluidic devices for nanoparticle preparation and electrochemical sensing. Devices were printed using poly(ethylene terephthalate) and featured threaded ports to connect polyetheretherketone (PEEK) tubing via printed fittings prepared from acrylonitrile butadiene styrene (ABS). These devices included channels designed to have 800 μm × 800 μm square cross sections and were semitransparent to allow visualization of the solution-filled channels. A 3D-printed device with a Y-shaped mixing channel was used to prepare Prussian blue nanoparticles (PBNPs) under flow rates of 100 to 2000 μL min(-1). PBNPs were then attached to gold electrodes for hydrogen peroxide sensing. 3D-printed devices used for electrochemical measurements featured threaded access ports into which a fitting equipped with reference, counter, and PBNP-modified working electrodes could be inserted. PBNP-modified electrodes enabled amperometric detection of H2O2 in the 3D-printed channel by flow-injection analysis, exhibiting a detection limit of 100 nM and linear response up to 20 μM. These experiments show that a consumer-grade FFF printer can be used to fabricate low-cost fluidic devices for applications similar to those that have been reported with more expensive 3D-printing methods.
使用消费级熔融沉积成型（FFF）3D打印机构建用于纳米颗粒制备和电化学传感的流体装置。装置采用聚对苯二甲酸乙二酯打印而成，并具有螺纹端口，以便通过由丙烯腈-丁二烯-苯乙烯（ABS）制备的打印配件连接聚醚醚酮（PEEK）管。这些装置包括设计为具有800μm×800μm方形横截面的通道，并且是半透明的，以便能够看到充满溶液的通道。一个带有Y形混合通道的3D打印装置用于在100至2000μL min(-1)的流速下制备普鲁士蓝纳米颗粒（PBNPs）。然后将PBNPs附着到金电极上用于过氧化氢传感。用于电化学测量的3D打印装置具有螺纹入口端口，可将配备有参比电极、对电极和PBNP修饰工作电极的配件插入其中。PBNP修饰电极通过流动注射分析能够对3D打印通道中的H2O2进行安培检测，检测限为100 nM，线性响应高达20μM。这些实验表明，消费级FFF打印机可用于制造低成本流体装置，用于与更昂贵的3D打印方法所报道的类似应用。
