Padeh S, Jaffe C L, Passwell J H
Samuel Jared Kushnick Pediatric Immunology Laboratory, Sheba Medical Center, Sackler School of Medicine, Rehovot, Israel.
Immunology. 1991 Feb;72(2):188-93.
We have studied the interaction of secretory immunoglobulin A (sIgA) derived from human breast milk with human monocytes. The presence of specific sIgA receptors on the monocyte membrane was confirmed by dose-dependent inhibition of E-sIgA rosette formation and by the binding of iodinated sIgA to monocyte monolayers. Binding was dependent on both the number of monocytes, as well as the amount of [125I]sIgA, and could be inhibited by unlabelled sIgA. Incubation of monocyte monolayers in the presence of increasing concentrations of secretory IgA and F(ab')2 anti-IgA resulted in a dose-dependent increase of the oxidative burst, as measured by H2O2 production. Neither sIgA or anti-IgA alone, nor incubation of IgG with anti-IgA, had any effect on the oxidative burst. These studies indicate that human monocytes have a receptor for sIgA and that specific activation of the monocytes occurs via these receptors.
我们研究了源自人母乳的分泌型免疫球蛋白A（sIgA）与人单核细胞的相互作用。通过剂量依赖性抑制E-sIgA玫瑰花结形成以及碘化sIgA与单核细胞单层的结合，证实了单核细胞膜上存在特异性sIgA受体。结合既取决于单核细胞的数量，也取决于[125I]sIgA的量，并且可被未标记的sIgA抑制。在存在浓度不断增加的分泌型IgA和F(ab')2抗IgA的情况下孵育单核细胞单层，通过H2O2产生量测定，导致氧化爆发呈剂量依赖性增加。单独的sIgA或抗IgA，以及IgG与抗IgA一起孵育，对氧化爆发均无任何影响。这些研究表明，人单核细胞具有sIgA受体，并且单核细胞通过这些受体发生特异性激活。
