Passwell J H, Schneeberger E, Merler E
Immunology. 1978 Dec;35(6):863-72.
The binding of sensitized red cells to Fc receptors in human monocytes was studied by evaluating the effects of various pharmacological reagents and other treatments on EA rosette formation. Cytochalasin B and 2-deoxyglucose inhibited rosette formation in a dose-dependent manner. Sodium azide and incubation at 4 degrees also inhibited rosette formation, while at 37 degrees increased numbers of RBCs bound to the monocytes. The microtubular poisons, vinblastine and colchicine at high concentrations resulted in decreased adherence of monocytes and inhibition of rosette formation, while at low concentrations of colchicine, enhanced rosette formation was sometimes observed. Contrary to the effects on rosette formation, binding of [125I] IgG to monocyte monolayers was not altered by treatment of the monocytes with drugs. Magnesium ions were required to promote monocyte adherence, but both magnesium and calcium were needed for the best rosette formation. We conclude that the formation of EA rosettes is dependent not merely on binding of IgG to the Fc receptor but requires metabolically active monocytes, an intact cytostructure and suitable environmental conditions (temperature and cation concentration).
通过评估各种药理试剂和其他处理对EA花环形成的影响，研究了致敏红细胞与人单核细胞中Fc受体的结合。细胞松弛素B和2-脱氧葡萄糖以剂量依赖的方式抑制花环形成。叠氮化钠和4℃孵育也抑制花环形成，而37℃时与单核细胞结合的红细胞数量增加。高浓度的微管毒物长春碱和秋水仙碱导致单核细胞黏附减少和花环形成受抑制，而低浓度秋水仙碱有时会观察到花环形成增强。与对花环形成的影响相反，用药物处理单核细胞不会改变[125I]IgG与单核细胞单层的结合。镁离子是促进单核细胞黏附所必需的，但最佳花环形成需要镁和钙两者。我们得出结论，EA花环的形成不仅依赖于IgG与Fc受体的结合，还需要代谢活跃的单核细胞、完整的细胞结构和合适的环境条件（温度和阳离子浓度）。
