Earhart Christopher M, Wilson Robert J, White Robert L, Pourmand Nader, Wang Shan X
Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305.
J Magn Magn Mater. 2009 May;321(10):1436-1439. doi: 10.1016/j.jmmm.2009.02.062.
A microfabricated magnetic sifter has been designed and fabricated for applications in biological sample preparation. The device enables high-throughput, high-gradient magnetic separation of magnetic nanoparticles by utilizing columnar fluid flow through a dense array (~5000/mm(2)) of micropatterned slots in a magnetically soft membrane. The potential of the sifter for separation of magnetic nanoparticles conjugated with capture antibodies is demonstrated through quantitative separation experiments with CD138-labelled MACS nanoparticles. Capture efficiencies ranging from 28-37% and elution efficiencies greater than 73% were measured for a single pass through the sifter.
一种用于生物样品制备的微制造磁选器已被设计并制造出来。该装置通过利用柱状流体流经磁性软膜中密集阵列(约5000个/平方毫米)的微图案化狭缝,实现了磁性纳米颗粒的高通量、高梯度磁分离。通过使用CD138标记的MACS纳米颗粒进行定量分离实验,证明了该选器用于分离与捕获抗体偶联的磁性纳米颗粒的潜力。单次通过选器时,测量到的捕获效率在28%-37%之间,洗脱效率大于73%。