寡核苷酸阳离子的电子转移解离
Electron Transfer Dissociation of Oligonucleotide Cations.
作者信息
Smith Suncerae I, Brodbelt Jennifer S
机构信息
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712.
出版信息
Int J Mass Spectrom. 2009 Jun 1;283(1-3):85-93. doi: 10.1016/j.ijms.2009.02.012.
Abstract
Electron transfer dissociation (ETD) of multi-protonated 6 - 20-mer oligonucleotides and 12- and 14-mer duplexes is compared to collision activated dissociation (CAD). ETD causes efficient charge reduction of the multi-protonated oligonucleotides in addition to limited backbone cleavages to yield sequence ions of low abundance. Subsequent CAD of the charge-reduced oligonucleotides formed upon electron transfer, in a net process termed electron transfer collision activated dissociation (ETcaD), results in rich fragmentation in terms of w, a, z, and d products, with a marked decrease in the abundance of base loss ions and internal fragments. Complete sequencing was possible for nearly all oligonucleotides studied. ETcaD of an oligonucleotide duplex resulted in specific backbone cleavages, with conservation of weaker non-covalent bonds.
摘要
将多质子化的6至20聚体寡核苷酸以及12聚体和14聚体双链体的电子转移解离（ETD）与碰撞激活解离（CAD）进行了比较。ETD除了导致有限的主链裂解以产生低丰度的序列离子外，还能使多质子化寡核苷酸有效地减少电荷。在一个称为电子转移碰撞激活解离（ETcaD）的净过程中，对电子转移后形成的电荷减少的寡核苷酸进行后续CAD，会产生丰富的w、a、z和d产物碎片，同时碱基丢失离子和内部碎片的丰度显著降低。几乎所有研究的寡核苷酸都可以进行完全测序。寡核苷酸双链体的ETcaD导致特定的主链裂解，同时较弱的非共价键得以保留。
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