Ferrick M R, Thurau S R, Oppenheim M H, Herbort C P, Ni M, Zachariae C O, Matsushima K, Chan C C
Howard Hughes Medical Institute, National Eye Institute, Bethesda, MD 20892.
Invest Ophthalmol Vis Sci. 1991 Apr;32(5):1534-9.
Interleukin-8 (IL-8), a cytokine with neutrophil chemotactic and activating properties, is known to be stimulated by IL-1. Fischer rats are more resistant to inflammation than Lewis rats probably due to a higher corticosteroid stress response. To determine the role of IL-8 in ocular inflammation, the effect of intravitreal injection of IL-8 was compared with that of IL-1 in both Lewis and Fischer rats. The IL-8, IL-1 alpha, or sterile balanced salt solution (control) was injected into one eye of each animal. Both IL-8 and IL-1 alpha caused inflammation in the eye of both strains, as detected by leukocyte counts of the anterior chamber and histopathologic examination. The eyes of animals injected with a cytokine had significantly higher numbers of leukocytes compared with eyes of control animals. Histopathologic examination confirmed these findings. The IL-1 alpha induced inflammation more consistently and more severely than the most effective dose of IL-8. This finding agreed with the concept of IL-1 initiating a cascade of inflammatory mediators including IL-8, which acts more specifically on a smaller population of leukocytes. A contralateral response was observed in the uninjected eye of experimental and control animals. The contralateral response in animals receiving the cytokines was significantly greater than that in controls. Lewis rats show a higher inflammatory response to the injections than do the Fischer rats. These data suggest that IL-8 may be active as one component in neutrophil-mediated ocular inflammation.
白细胞介素-8（IL-8）是一种具有中性粒细胞趋化和激活特性的细胞因子，已知可被IL-1刺激。Fischer大鼠比Lewis大鼠对炎症更具抵抗力，这可能是由于其更高的皮质类固醇应激反应。为了确定IL-8在眼部炎症中的作用，在Lewis大鼠和Fischer大鼠中比较了玻璃体内注射IL-8与IL-1的效果。将IL-8、IL-1α或无菌平衡盐溶液（对照）注射到每只动物的一只眼睛中。通过前房白细胞计数和组织病理学检查发现，IL-8和IL-1α均在两种品系的眼中引起炎症。与对照动物的眼睛相比，注射细胞因子的动物的眼睛中白细胞数量明显更高。组织病理学检查证实了这些发现。IL-1α诱导的炎症比最有效剂量的IL-8更持续、更严重。这一发现与IL-1引发包括IL-8在内的一系列炎症介质的概念一致，IL-8对较少数量的白细胞具有更特异性的作用。在实验动物和对照动物未注射的眼睛中观察到对侧反应。接受细胞因子的动物的对侧反应明显大于对照组。Lewis大鼠对注射的炎症反应比Fischer大鼠更高。这些数据表明，IL-8可能作为中性粒细胞介导的眼部炎症的一个组成部分发挥作用。
