叶黄素和玉米黄质对脂多糖诱导的葡萄膜黑素细胞分泌白细胞介素-8的影响及相关信号通路
Effects of Lutein and Zeaxanthin on LPS-Induced Secretion of IL-8 by Uveal Melanocytes and Relevant Signal Pathways.
作者信息
Chao Shih-Chun, Vagaggini Tommaso, Nien Chan-Wei, Huang Sheng-Chieh, Lin Hung-Yu
机构信息
Department of Ophthalmology, Show Chwan Memorial Hospital, No. 526, Sec. 1, Zhongshan Road, Changhua 500, Taiwan ; Institute of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan ; Central Taiwan University of Science and Technology, No. 666, Buzih Road, Beitun District, Taichung 40601, Taiwan.
Albert Einstein College of Medicine, Yeshiva University, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
出版信息
J Ophthalmol. 2015;2015:152854. doi: 10.1155/2015/152854. Epub 2015 Nov 2.
The effects of lutein and zeaxanthin on lipopolysaccharide- (LPS-) induced secretion of IL-8 by uveal melanocytes (UM) were tested in cultured human UM. MTT assay revealed that LPS (0.01-1 μg/mL) and lutein and zeaxanthin (1-10 μM) did not influence the cell viability of cultured UM. LPS caused a dose-dependent increase of secretion of IL-8 by cultured UM. Lutein and zeaxanthin did not affect the constitutive secretion of IL-8. However, lutein and zeaxanthin decreased LPS-induced secretion of IL-8 in cultured UM in a dose-dependent manner. LPS significantly increased NF-κB levels in cell nuclear extracts and p-JNK levels in the cell lysates from UM, but not p-p38 MAPK and p-ERG. Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels. The present study demonstrated that lutein and zeaxanthin inhibited LPS-induced secretion of IL-8 in cultured UM via JNK and NF-κB signal pathways. The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.
在培养的人葡萄膜黑色素细胞（UM）中测试了叶黄素和玉米黄质对脂多糖（LPS）诱导的UM分泌白细胞介素-8（IL-8）的影响。MTT法显示，LPS（0.01 - 1μg/mL）以及叶黄素和玉米黄质（1 - 10μM）不影响培养的UM的细胞活力。LPS导致培养的UM分泌IL-8呈剂量依赖性增加。叶黄素和玉米黄质不影响IL-8的组成性分泌。然而，叶黄素和玉米黄质以剂量依赖性方式降低了培养的UM中LPS诱导的IL-8分泌。LPS显著增加了UM细胞核提取物中的NF-κB水平以及细胞裂解物中的p-JNK水平，但未增加p-p38 MAPK和p-ERG水平。叶黄素或玉米黄质显著降低了LPS诱导的NF-κB和p-JNK水平的升高，但未降低p38 MAPK和ERG水平。本研究表明，叶黄素和玉米黄质通过JNK和NF-κB信号通路抑制培养的UM中LPS诱导的IL-8分泌。叶黄素和玉米黄质的抗炎作用可能作为葡萄膜炎和其他眼部炎症性疾病治疗方法进行探索。
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