Institute of Pathogens and Vectors, Dali University, Dali, China.
PLoS One. 2010 Feb 11;5(2):e9167. doi: 10.1371/journal.pone.0009167.
Influenza A virus displays strong reassortment characteristics, which enable it to achieve adaptation in human infection. Surveying the reassortment and virulence of novel viruses is important in the prevention and control of an influenza pandemic. Meanwhile, studying the mechanism of reassortment may accelerate the development of anti-influenza strategies.
METHODOLOGY/PRINCIPAL FINDINGS: The hemagglutinin (HA) and neuraminidase (NA) matching patterns of two pandemic H1N1 viruses (the 1918 and current 2009 strains) and a highly pathogenic avian influenza A virus (H5N1) were studied using a pseudotyped particle (pp) system. Our data showed that four of the six chimeric HA/NA combinations could produce infectious pps, and that some of the chimeric pps had greater infectivity than did their ancestors, raising the possibility of reassortment among these viruses. The NA of H5N1 (A/Anhui/1/2005) could hardly reassort with the HAs of the two H1N1 viruses. Many biological characteristics of HA and NA, including infectivity, hemagglutinating ability, and NA activity, are dependent on their matching pattern.
CONCLUSIONS/SIGNIFICANCE: Our data suggest the existence of an interaction between HA and NA, and the HA NA matching pattern is critical for valid viral reassortment.
甲型流感病毒具有很强的重配特征，使其能够在人类感染中实现适应。调查新型病毒的重配和毒力对于流感大流行的预防和控制非常重要。同时，研究重配的机制可能会加速抗流感策略的发展。
方法/主要发现：我们使用假型颗粒（pp）系统研究了两种大流行的 H1N1 病毒（1918 年和当前的 2009 年株）和一种高致病性禽流感病毒（H5N1）的血凝素（HA）和神经氨酸酶（NA）匹配模式。我们的数据表明，六种嵌合 HA/NA 组合中的四种可以产生感染性 pp，并且一些嵌合 pp 的感染性比其祖先更强，这增加了这些病毒之间发生重配的可能性。H5N1（A/Anhui/1/2005）的 NA 几乎不能与两种 H1N1 病毒的 HA 重配。HA 和 NA 的许多生物学特性，包括感染力、血凝能力和 NA 活性，都取决于它们的匹配模式。
结论/意义：我们的数据表明 HA 和 NA 之间存在相互作用，HA-NA 匹配模式对于有效的病毒重配至关重要。
